大鼠背根神经节持续受压后膜联蛋白A2表达及分布的变化  

Expression and distribution of annexin A2 in dorsal root ganglion after chronic compression of dorsal root ganglion in rats

作　　者：宁丽萍 范晓华 王传伟[2] 韩君婷 丁欣利 王超 刘玉光[2] Ning Liping;Fan Xiaohua;Wang Chuanwei;Han Junting;Ding Xinli;Wang Chao;Liu Yuguang(Department of Rehabilitation Medicine,Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;Department of Neurosurgery,Qilu Hospital Affiliated to Shandong University,Jinan 250012,China)

机构地区：[1]山东第一医科大学附属省立医院康复医学科,济南250021 [2]山东大学齐鲁医院神经外科,济南250012

出　　处：《中华神经医学杂志》2021年第9期880-885,共6页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金(81401868、81502148);山东省重点研发计划项目(2016GSF201069)。

摘　　要：目的探讨大鼠背根神经节(DRG)持续受压(CCD)后DRG中膜联蛋白A2(ANXA2)的表达及分布规律。方法成年雄性Wistar大鼠102只按随机数字表法分为对照组(24只)、CCD模型后7 d组(30只)、CCD模型后14 d组(24只)及CCD模型后28 d组(24只)。后3组大鼠采用"U"形棒制备CCD模型。采用机械痛刺激仪和热痛刺激仪测量各组大鼠的机械缩足反射阈值(MWT)和热辐射缩爪反应潜伏期(TWL)。采用Western blotting和免疫荧光染色检测各组大鼠DRG中ANXA2蛋白的表达。采用免疫荧光双标染色检测CCD模型后7 d组大鼠DRG中ANXA2和神经元标志物微管蛋白β3(TUBB3)的表达。结果与对照组比较,CCD模型后7 d组、CCD模型后14 d组大鼠的MWT与TWL均明显下降,差异均有统计学意义(P<0.05)。Western blotting检测显示,与对照组比较,CCD模型后7 d组大鼠DRG中ANXA2蛋白的表达升高,差异有统计学意义(P<0.05)。免疫荧光染色检测显示CCD模型后7 d组大鼠DRG中ANXA2的免疫反应性较对照组增强。免疫荧光双标染色检测显示CCD模型后7 d组大鼠DRG中ANXA2主要表达于神经元的细胞膜中。结论CCD模型大鼠的机械痛阈与热痛阈下降,受压侧DRG内ANXA2蛋白表达上调及免疫反应性增强,ANXA2可能参与CCD后神经病理性疼痛的发生发展。Objective To explore the annexin A2(ANXA2)expression and distribution in dorsal root ganglion(DRG)after chronic compression of DRG(CCD)in rat models.Methods One hundred and two adult male Wistar rats were randomly divided into control group(n=24),CCD model group A(7 d after modeling,n=30),CCD model group B(14 d after modeling,n=24),and CCD model group D(28 d after modeling,n=24).Rats in the later 3 groups were established CCD models with the help of"U"rod screw.Mechanical withdrawal threshold(MWT)and thermal radiation paw withdrawal latency(TWL)were measured by mechanical pain stimulator and thermal pain stimulator.The ANXA2 protein expression in the DRG was detected by Western blotting and immunofluorescent staining.The distributions of ANXA2 and class IIIβ-tubulin(TUBB3)positive cells in DRG were detected by immunofluorescence double staining.Results As compared with those in the control group,MWT and TWL in the CCD model group A and CCD model group B were significantly decreased(P<0.05).Western blotting showed that ANXA2 protein expression in the DRG of CCD model group A was statistically increased as compared with that in the control group(P<0.05).Immunofluorescent staining showed that the immunoreactivity of ANXA2 in DRG of CCD model group A was enhanced as compared with that in control group.Immunofluorescence double staining showed that ANXA2 was mainly expressed in the cell membrane of neurons in the DRG of CCD model group A.Conclusion The mechanical and thermal pain thresholds are decreased,while the ANXA2 protein expression at the pressure side of DRG is up-regulated and the immunoreactivity is increased in CCD models;ANXA2 may be involved in the occurrence and development of pathological neuralgia after CCD.

关 键 词：背根神经节 膜联蛋白A2 持续受压 神经病理性疼痛 

分 类 号：R338[医药卫生—人体生理学]