玉米籽粒相关性状的QTL定位  

QTLs Mapping for Traits Related with Kernel in Maize

作　　者：王新涛 李保叶 杨青 代资举 郝俊杰[1] WANG Xintao;LI Baoye;YANG Qing;DAI Ziju;HAO Junjie(Plant Protection Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Henan Academy of Crop Molecular Breeding,Zhengzhou 450002,China)

机构地区：[1]河南省农业科学院植物保护研究所,河南郑州450002 [2]河南省农业科学院芝麻研究中心,河南郑州450002 [3]河南省作物分子育种研究院,河南郑州450002

出　　处：《河南农业科学》2021年第9期9-15,共7页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关计划项目(192102110026,202102110155);河南省农业科学院科研发展专项(2020CY011,2020YQ33)。

摘　　要：为探究玉米籽粒百粒质量、粒长和粒宽的遗传机制,以郑58×D863F构建的包含241个家系的重组自交系(RIL)群体为材料,进行2a不同环境下的百粒质量、粒长和粒宽QTL定位分析。结果表明,玉米百粒质量、粒长和粒宽在RIL群体中表现出连续变异,呈正态分布,且性状间呈极显著相关,属于典型的数量性状。在2 a不同环境下共检测到14个QTL,其中,控制百粒质量、粒长和粒宽的QTL分别有6个、3个和5个,分布在第2、3、4、7染色体上,表型贡献率介于6.42%~13.41%。其中,位于第2染色体的控制百粒质量的QTL q100KW2连续2 a的表型贡献率均大于10%,为主效QTL;在第4染色体umc1891—mmc0371区间内同时检测到控制百粒质量、粒长和粒宽的QTL,这些QTL可能包含调控籽粒性状的关键基因。In order to study the genetic mechanism of 100-kernel weight,kernel length and kernel width in maize,a recombinant inbred line(RIL)population including 241 lines derived from Zheng 58×D863F was used as material,and QTLs for 100-kernel weight,kernel length and kernel width were studied from^(2)018 to 2019 under two different environments.In RIL population,100-kernel weight,kernel length and kernel width showed continuous variations with a normal distribution and significant correlations,and belonged to typical quantitative traits.A total of 14 QTLs were detected in two years under two different environments.The numbers of QTLs for 100-kernel weight,kernel length and kernel width were 6,3 and 5,respectively.These QTLs were detected on chromosomes 2,3,4 and 7,and the PVE(phenotypic variance explained)values were 6.42%—13.41%.The PVE values of major QTL(q100KW2)for 100-kernel weight detected on chromosome 2 were above 10%under two environments.The QTLs for 100-kernel weight,kernel length and kernel width were detected in umc1891—mmc0371 on chromosome 4,which might contain key genes regulating grain traits.

关 键 词：玉米 重组自交系群体 QTL 百粒质量 粒长 粒宽 

分 类 号：S513[农业科学—作物学]