61份高油大豆品种(系)指纹图谱构建及遗传多样性分析  

Construction of Fingerprint and Genetic Diversity Analysis of 61 High-oil Soybean Varieties(Lines)

作　　者：李琼 李金花 常世豪 杨青春 舒文涛 耿臻 LI Qiong;LI Jinhua;CHANG Shihao;YANG Qingchun;SHU Wentao;GENG Zhen(Zhoukou Academy of Agricultural Sciences,Zhoukou 466001,China)

机构地区：[1]周口市农业科学院,河南周口466001

出　　处：《河南农业科学》2021年第9期53-62,共10页Journal of Henan Agricultural Sciences

基　　金：河南省现代农业产业技术体系甘薯杂粮体系大豆育种岗位专家项目(S2020-14-G01)。

摘　　要：以61份高油分含量的大豆品种(系)为供试材料,选择68对SSR标记引物检测高油大豆品种(系)的遗传多样性并建立指纹图谱,为高油大豆的亲本选配、品种鉴定提供理论依据。结果显示,61份高油性指数(H)为0.272 4,平均Shannon’s信息指数(I)为0.429 7,遗传多样性较为丰富。聚类分析结果表明,61份材料的遗传相似系数(GS)为0.61~0.87,在遗传相似系数0.63处,61份高油大豆材料被分为四大品种(系),并构建了供试材料的指纹图谱。将61份高油大豆材料按照产地来源及种植生态区分为群体A(来自辽宁、黑龙江、山西、河北、北京)、群体B(来自河南、山东、安徽、江苏、湖北)、群体C(美国引进),0.250 2,群体间遗传分化系数(Gst)为0.096 4,基因流(Nm^(*))为4.688 7。群体间存在低度遗传分化,遗传变异主要存在于群体内部,群体间基因交流丰富。由3个群体之间的遗传距离比较可知,群体B与群体C亲缘关系最远,群体A与群体C亲缘关系居中,群体A与群体B亲缘关系最近。Sixty-one soybean varieties(lines)of high-oil content were used as test materials,and 68 pairs of SSR markers were selected to detect the genetic diversity of high-oil soybean varieties(lines)and establish fingerprint,which would provide a theoretical basis for parent selection and variety identification of high-oil soybeans.The results of high-oil soybean materials(61 copies)showed that the average number of alleles(Na)was 1.9803,the average number of effective alleles(Ne)was 1.4142,the average number of Nei’s gene diversity index(H)was 0.2724,and the average number of Shannon’s information index(I)was 0.4297.The genetic diversity of high-oil soybean materials(61 copies)is relatively rich.The clustering results showed that the genetic similarity coefficient(GS)of 61 materials was between 0.61 and 0.87,and at the genetic similarity coefficient of 0.63,61 materials were divided into four major groups.In this study,a total of seven pairs of core primers(sat304,satt708,satt373,satt216,satt463,satt631,satt268)were screened to effectively distinguish the varieties(lines),and the fingerprints of the tested materials were constructed.According to origin and planting ecological area,the61 materials could be divided into three groups.The materials of group A were from Liaoning,Heilongjiang,Shanxi,Hebei,Beijing.The materials of group B were from Henan,Shandong,Anhui,Jiangsu,Hubei.The materials of group C were introduced from the United States.The genetic diversity of three groups was A>B>C.The total genetic diversity(Ht)among the three groups was 0.2769,the genetic diversity within the groups(Hs)was 0.2502,the coefficient of genetic differentiation(Gst)among the groups was 0.0964,and the gene flow(Nm^(*))was 4.6887.There was a low degree of genetic differentiation among groups,genetic variation mainly existed within groups,and gene flow among groups was abundant.According to the comparison of genetic distances among the three groups,group B had the farthest genetic relationship with group C,group A had the middle genet

关 键 词：高油大豆 种质资源 遗传多样性 SSR 指纹图谱 

分 类 号：S565.1[农业科学—作物学]