人体肝功能单元结构三维构筑的初步探索  

Preliminary exploration of architecture of human hepatic functional unit

作　　者：杨静怡 刘红军 陈娜[1] 侯宇丰 吕沄禧 李其 梅峰[4] 平轶芳 郑璐[6] 吴毅[1] YANG Jingyi;LIU Hongjun;CHEN Na;HOU Yufeng;LYU Yunxi;LI Qi;MEI Feng;PING Yifang;ZHENG Lu;WU Yi(Department of Digital Medicine,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing,400038;Squadron 15,Team 5,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing,400038;Squadron 17,Team 5,College of Biomedical Engineering and Medical Imaging,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing,400038;Department of Histology and Embryology,College of Basic Medical Sciences,Army Medical University(Third Military Medical University),Chongqing,400038;Institute of Pathology,First Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,400038;Department of Hepatobilliary Surgery,Second Affiliated Hospital,Army Medical University(Third Military Medical University),Chongqing,400037,China)

机构地区：[1]陆军军医大学(第三军医大学)生物医学工程与影像医学系数字医学教研室,重庆400038 [2]陆军军医大学(第三军医大学)基础医学院学员五大队十五中队,重庆400038 [3]陆军军医大学(第三军医大学)生物医学工程与影像医学五大队十七队,重庆400038 [4]陆军军医大学(第三军医大学)基础医学院组织胚胎学教研室,重庆400038 [5]陆军军医大学(第三军医大学)附属第一医院病理科,重庆400038 [6]陆军军医大学(第三军医大学)附属第二医院肝胆科,重庆400037

出　　处：《第三军医大学学报》2021年第18期1713-1719,共7页Journal of Third Military Medical University

基　　金：国家自然科学基金面上项目(31671251);重庆英才青年拔尖人才项目(CQYC201905037);陆军科技英才(91003201901973)。

摘　　要：目的通过数字化技术三维构筑人体肝功能单元,并对其三维形态和空间毗邻关系进行研究,以了解肝功能单元肝细胞的三维构筑规律。方法选择人体肝组织,通过常规石蜡连续切片、HE染色和显微镜扫描成像,获取4μm厚度的肝组织学切片,使用Photoshop软件对切片染色图像进行配准,使用AMIRA软件对肝功能单元包括肝小叶轮廓、肝血窦、肝细胞索、中央静脉、小叶间动脉、静脉和胆管进行三维重建,对其进行组织形态学参数测量和研究。结果构筑了肝功能单元的三维数字化模型,结果显示肝小叶外轮廓为不规则的较圆钝的多边形结构,边长不等,最大直径可达1495.27μm,最小直径为1036.96μm;肝小叶的中央静脉并不位于肝小叶正中,依次表现为322.38、474.05、878.7、880.16μm;肝血窦为相互交织的血管网,并不构成放射状排列的血管网。结论本研究中的三维重建图能够精准反映肝功能单元的三维形态和空间毗邻关系,为建立肝脏微血管系统提供研究基础。Objective To construct a three-dimensional of human hepatic functional unit through digital technology,and study its 3 D shape and spatial adjoining relationship in order to understand the construction law of hepatic functional unit hepatic lobule cells.Methods We selected human hepatic lobule tissue,and used conventional paraffin serial sections,HE staining technology and microscope scanning to obtain hepatic lobule section in 4-μm thickness.We employed Photoshop software for image registration and used AMIRA software 2019.1 version for 3 D reconstruction of hepatic functional unit which include hepatic lobule.Lobular contours,hepatic sinusoids,hepatocyte cords,central veins,interlobular arteries,veins and bile ducts were measured and studied for the tissue morphological parameters.Results A 3 D digital model of the hepatic functional unit was reconstructed,and we founded that the contour of the hepatic lobule was irregularly round and blunt polygon structure with different edge lengths.The maximum diameter was 1495.27μm and the minimum diameter was 1036.96μm;The central vein of the hepatic lobule was not located in the middle of the hepatic lobule,at 322.38,474.05,878.7 and 880.16μm,The hepatic sinusoids were interwoven vascular networks,which did not constitute a radial arrangement of blood vessel networks.Conclusion The results of our 3 D morphological study of hepatic lobule are different from the traditional anatomy textbooks and atlas,which not only update our knowledge of the structure of hepatic functional unit,and provide a 3 D visualization image of the real human hepatic lobule structure.However,we think that the 3 D morphology of hepatic functional unit structure is produced under the condition of hepatic lobule cell shaping development and reshaping redevelopment,therefore it provides a computer model for the 3 D printing of hepatic lobule tissue cells.

关 键 词：肝小叶 肝功能单元 三维重建 三维构筑 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329-3[医药卫生—基础医学]