三种基因在非小细胞肺癌外周血有核细胞中阳性表达的意义  

Significance of positive expression of three genes in peripheral blood nucleated cells of non-small cell lung cancer

作　　者：农雪萍 NONG Xue-ping(Department of Pathology,Jiangxi Chest Hospital,Jiangxi Province,Nanchang 330006,China)

机构地区：[1]江西省胸科医院病理科,江西南昌330006

出　　处：《中国当代医药》2021年第27期120-122,126,共4页China Modern Medicine

基　　金：江西省卫生计生委科技计划项目(20175512)。

摘　　要：目的研究非小细胞肺癌患者的肺组织特异性蛋白、细胞角蛋白19、钙结合蛋白A4三种基因在外周血液有核细胞中的阳性表达情况。方法选择2018年3月至2019年12月在江西省胸科医院接受治疗的肺部良性疾病和非小细胞肺癌确诊患者各60例,分别作为研究1组和研究2组,再抽取同期在江西省胸科医院接受健康体检的同年龄段健康人资料60例作为对照组。采用荧光定量PCR检测法对三组研究对象的肺组织特异性蛋白、细胞角蛋白19、钙结合蛋白A4三种基因在外周血液有核细胞中的阳性表达情况进行测定,并比较检测结果。结果研究1组肺组织特异性蛋白以及钙结合蛋白水平高于对照组,差异有统计学意义(P<0.05);研究2组肺组织特异性蛋白水平高于研究1组,差异有统计学意义(P<0.05);研究2组的肺组织特异性蛋白、细胞角蛋白19、钙结合蛋白A4三种基因在外周血液有核细胞中表达的阳性率高于研究1组,差异有统计学意义(P<0.05);研究1组的肺组织特异性蛋白、细胞角蛋白19、钙结合蛋白A4三种基因在外周血液有核细胞中表达的阳性率高于对照组,差异有统计学意义(P<0.05)。结论非小细胞肺癌患者的肺组织特异性蛋白、细胞角蛋白19、钙结合蛋白A4三种基因在外周血液有核细胞中表达的阳性率会明显高于肺部良性疾病患者及健康人群,临床上可以将这一特征作为非小细胞肺癌诊断和治疗效果评价的重要参考依据。Objective To study the expression of lung tissue-specific protein,cytokeratin 19 and calcium binding protein A4 in peripheral blood nucleated cells of patients with non-small cell lung cancer.Methods Each of 60 patients with confirmed lung benign and non-small cell lung cancer treated at Jiangxi Chest Hospital from March 2018 to December 2019 were respectively selected as study group 1 and study group 2.Then 60 healthy people of the same age who received physical examination in Jiangxi Chest Hospital during the same period were selected as the control group.The positive expression of lung tissue-specific protein,cytokeratin 19 and calcium binding protein A4 in peripheral blood nucleated cells of the three groups were detected by fluorescence quantitative PCR,and the detection results were compared.Results The levels of lung tissue-specific protein and calcium binding protein in the study 1 group were higher than those in the control group,the differences were statistically significant(P<0.05).The level of lung tissue-specific protein in the study 2 group was higher than that in the study 1 group,the difference was statistically significant(P<0.05).Three genes of expression of lung tissue-specific protein,cytokeratin 19,and calcium-binding protein A4 in peripheral blood nucleocytes in the study 2 group were higher than that in the study 1 group,the differences were statistically significant(P<0.05).Three genes of expression of lung tissue-specific protein,cytokeratin 19,and calcium-binding protein A4 in peripheral blood nucleocytes in the study 1 group were higher than that in the control group,the differences were statistically significant(P<0.05).Conclusion The positive rates of lung tissue-specific protein,cytokeratin 19 and calcium binding protein A4 in peripheral blood nucleated cells of patients with nonsmall-cell lung cancer are significantly higher than those of patients with benign lung diseases and healthy people,which could be used as an important reference for the diagnosis and treatment of NSCLC.

关 键 词：非小细胞肺癌 肺组织特异性蛋白 细胞角蛋白19 钙结合蛋白A4 外周血液 有核细胞 阳性表达 

分 类 号：R642[医药卫生—外科学]