高产β-甘露聚糖酶菌株的分离鉴定及酶学性质研究  

Isolation and identification of β-mannanase-producing strain and its enzymatic property

作　　者：陈晓飞[1,2] 李珊珊[1,2] 刁文涛[1,2] 徐紫瑜 刘德海[1,2] CHEN Xiaofei;LI Shanshan;DIAO Wentao;XU Ziyu;LIU Dehai(Institute of Biology Co.,Ltd.,Henan Academy of Science,Zhengzhou 450008,China;Key Laboratory of Microbial Engineering of Henan Province,Zhengzhou 450008,China;School of Life Sciences,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南省科学院生物研究所有限责任公司,河南郑州450008 [2]河南省微生物工程重点实验室,河南郑州450008 [3]河南农业大学生命科学学院,河南郑州450046

出　　处：《中国酿造》2021年第9期92-97,共6页China Brewing

基　　金：中原科技创新领军人才计划项目(214200510011)。

摘　　要：通过初筛和复筛从魔芋种植基地土壤样品中分离筛选高产β-甘露聚糖酶菌株。通过形态学观察、生理生化试验及16S rDNA序列分析对菌株进行鉴定,并对其产β-甘露聚糖酶酶学性质进行研究。结果表明,筛选出一株高产β-甘露聚糖酶的菌株,编号为HTGC-10,被鉴定为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。菌株HTGC-10在30℃、180 r/min条件下液体发酵24 h后,发酵液β-甘露聚糖酶活力为61.75 U/mL。酶学性质的研究结果表明,该菌株所产酶的最适反应pH值为6.0,在中性偏酸环境下稳定性较好,属于偏酸性酶;最适反应温度为55℃,热稳定性相对较差;乙二胺四乙酸(EDTA)和金属离子 Na^(+)、K^(+)、Mn^(2+)、NH_(4)^(+)、Mg^(2+)、Fe^(2+)、Cu^(2+)、Ca^(2+)对酶活力均有不同程度的抑制作用,其中Cu^(2+)对酶活力的抑制作用最显著。High-yieldβ-mannanase strain was isolated and screened from soil samples of Konjac planting base through primary and secondary screening.The strains were identified by morphological observation,physiological and biochemical tests and 16S rDNA sequence analysis,and the enzymatic properties ofβ-mannanase produced by the strain were studied.The results showed that a high-yieldβ-mannanase strain was screened out,namely HTGC-10,which was identified as Bacillus amyloliquefaciens.Theβ-mannanase activity of fermentation broth was 61.75 U/ml after liquid fermentation of strain HTGC-10 at 30℃and 180 r/min for 24 h.The results of enzymatic properties showed that the optimal reaction pH value of the enzyme produced by the strain was 6.0,and it was stable in neutral to acidic environment,and belonged to the acidic enzyme.The optimal reaction temperature was 55℃,and the thermal stability was relatively poor.Ethylene diamine tetraacetic acid(EDTA)and metal ions Na^(+),K^(+),Mn^(2+),NH_(4)^(+),Mg^(2+),Fe^(2+),Cu^(2+),Ca^(2+)showed different degree inhibition effect on enzyme activity,of which Cu^(2+)showed the most significant inhibition effect on enzyme activity.

关 键 词：Β-甘露聚糖酶 分离 鉴定 酶学性质 

分 类 号：S816.3[农业科学—饲料科学]