新型无酶信号放大技术在生物传感器中的应用  

Novel enzyme-free signal amplification strategies applied in biosensors

作　　者：杨建梅 向云[1] Yang Jian-mei;Xiang Yun(College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《化学传感器》2019年第3期10-21,共12页Chemical Sensors

基　　金：国家自然科学基金资助项目(21675128)。

摘　　要：为实现低含量生物分子的检测需求,常常需要借助信号放大技术来提高传感器的灵敏度。现有的信号放大技术大致可以分为酶辅助的信号放大及无酶信号放大这两类。酶固有的稳定性差、反应条件苛刻等缺点使酶辅助信号放大技术在应用上受到了一定的限制。无酶信号放大技术则不需要苛刻的实验条件且操作简单,因此受到了人们的广泛关注。该文主要就新型无酶信号放大技术在生物传感器中的应用作一综述。In order to achieve the detection requirements of low content biomolecules,it is often necessary to use signal amplification strategies to improve the sensitivity of the biosensors.Current signal amplification strategies can be divided into two categories:enzyme-assisted signal amplification and enzyme-free signal amplification.The inherent disadvantages of enzyme,such as poor stability and harsh reaction conditions,limit the application of enzyme-assisted signal amplification.The enzyme-free signal amplification strategy has arisen worldwide concerns because it does not need cumbersome procedures and it is easy to operate.This review discusses the application of the enzyme-free amplification in biosensors.

关 键 词：无酶信号放大 生物传感器 纳米材料 链置换反应 DNA自组装 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]