不同营养条件下甜菜夜蛾幼虫感染核型多角体病毒的敏感性及取食趋性  

Sensitivity of Spodoptera exigua larvae to nucleopolyhedrovirus and their feeding preference under different nutritional conditions

作　　者：乔风娇 邢龙生 李长友 郑桂玲 张彬 QIAO Feng-Jiao;XING Long-Sheng;LI Chang-You;ZHENG Gui-Ling;ZHANG Bin(College of Plant Health&Medicine,Qingdao Agricultural University,Shandong Province Key Laboratory for Integrated Control of Plant Diseases and Pest Pests,Sino-Australian Joint Research Institute of Agriculture and Environmental Health,Qingdao 266109,Shandong Province,China;Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Genomics Institute Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,Guangdong Province,China)

机构地区：[1]青岛农业大学植物医学学院,山东省植物病虫害综合防控重点实验室,中澳农业与环境健康联合研究院,山东青岛266109 [2]岭南现代农业科学与技术广东省实验室深圳分中心,农业农村部农业基因数据分析重点实验室,中国农业科学院(深圳)农业基因组研究所,深圳518120

出　　处：《环境昆虫学报》2021年第5期1122-1128,共7页Journal of Environmental Entomology

基　　金：国家自然科学基金(31972333);山东省自然科学基金(ZR2020MC128);深圳市基础研究面上项目(JCYJ20190813144407666)。

摘　　要：为探讨不同营养条件下甜菜夜蛾Spodoptera exigua对甜菜夜蛾核型多角体病毒(Spodoptera exigua multiple nucleopolyhedrovirus,SeMNPV)的敏感性及取食行为响应,本研究通过改变甜菜夜蛾人工饲料配方建立高蛋白低糖(简称高蛋白)、中蛋白中糖(简称中蛋白)、低蛋白高糖(简称低蛋白)3种营养品系,观察不同营养品系幼虫对SeMNPV的敏感性,以及染毒前后对不同营养饲料的取食趋性。结果显示,高蛋白、中蛋白、低蛋白3种营养品系中,SeMNPV对甜菜夜蛾初孵幼虫的半数致死浓度(LC_(50))分别是1.76×10^(5)PIB/mL、2.85×10^(5)PIB/mL和2.10×10^(4)PIB/mL。统计分析显示,中蛋白品系的LC_(50)显著高于低蛋白品系,高蛋白品系的LC_(50)同其余两种品系相比无显著性差异。以8×10^(5)PIB/mL浓度的病毒饲喂甜菜夜蛾初孵幼虫,3种营养品系的半数致死时间(LT 50)分别是6.07 d、6.85 d和5.20 d,中蛋白品系的存活时间显著长于低蛋白品系,高蛋白品系的LT 50同其余两种品系相比无显著性差异。未染毒状态下,低龄幼虫偏向蛋白质含量较高的饲料,而高龄幼虫则偏向于高糖的饲料;染毒状态下,甜菜夜蛾各龄期幼虫都偏向于取食蛋白质含量较高的饲料。以上研究结果表明,营养条件的变化可以显著影响甜菜夜蛾幼虫对SeMNPV的敏感性,染毒后幼虫明显偏向于蛋白质含量高的饲料,推测蛋白质可能有助于提高甜菜夜蛾幼虫对SeMNPV的抵抗力。To study the sensitivity of Spodoptera exigua larvae to Spodoptera exigua multiple nucleopolyhedrovirus(SeMNPV)infection and their feeding behavior response under different nutritional conditions,three nutritional colonies of S.exigua were established in this study by changing the formula of artificial diet,including high protein and low carbohydrate(referred to as high protein),moderate protein and moderate carbohydrate(referred to as moderate protein),low protein and high carbohydrate(referred to as low protein).Subsequently,we observed of the larvae of different nutritional colonies to SeMNPV and their feeding preference on different nutritional feeds before and after virus infection.The result showed that the median lethal concentration(LC_(50))of three colonies of S.exigua larvae was 1.76×10^(5)PIB/mL,2.85×10^(5)PIB/mL and 2.10×10^(4)PIB/mL,respectively.Among them,the LC_(50)of the moderate protein colony was significantly higher than that of the low protein colony.After administered with SeMNPV at a concentration of 8×10^(5)PIB/mL,the median lethal time(LT 50)three colonies of S.exigua larvae was 6.07 d,6.85 d and 5.20 d,respectively.The LT 50 of the moderate protein colony was significantly longer than that of the low protein colony.For uninfected S.exigua larvae,young larvae(before 3 rd instar)preferred the foods with high protein content,while older larvae tend to eat high carbohydrate foods.However,the infected larvae,across the whole larval stage were inclined to eat high protein foods.The above results suggested that the change of nutritional conditions could significantly affect the sensitivity of S.exigua larvae to SeMNPV,and the larvae obviously preferred the diet with high protein content after infection with the virus.It was speculated that protein might help to improve the resistance of S.exigua larvae to SeMNPV.

关 键 词：营养 核型多角体病毒 甜菜夜蛾 毒力 取食趋性 

分 类 号：Q963[生物学—昆虫学] S476[农业科学—农业昆虫与害虫防治]