番茄褪绿病毒对Q型烟粉虱重要生物学参数及保护酶和解毒酶活力的影响  

Impact of ToCV on important biological parameters,and activities of protective and detoxification enzymes in Bemisia tabaci Q

作　　者：刘国霞[1,2] 李娇娇[1] 张安盛[1] LIU Guo-Xia;LI Jiao-Jiao;ZHANG An-Sheng(Plant Protection Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China;Biotechnology Research Center,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]山东省农业科学院植物保护研究所,济南250100 [2]山东省农业科学院生物技术研究中心,济南250100

出　　处：《环境昆虫学报》2021年第5期1237-1243,共7页Journal of Environmental Entomology

基　　金：山东省现代农业产业技术体系烟草产业创新团队(SDAIT-25-03)。

摘　　要：为明确番茄褪绿病毒(ToCV)对其传毒介体烟粉虱Bemisia tabaci主要生物学特性的影响,本文研究了携带ToCV的Q型烟粉虱在非病毒寄主植物棉花Gossypium spp上的生物学指标,并测定了带毒和无毒烟粉虱主要保护酶和解毒酶活性。结果表明,在棉花植株上,带毒烟粉虱在发育历期、产卵量、成虫寿命方面与无毒烟粉虱无显著差异,但雌虫体长明显短于无毒烟粉虱。相对于无毒烟粉虱,带毒烟粉虱体内过氧化氢酶(catalase,CAT)活性明显提高,是无毒烟粉虱的3.36倍(P<0.001),超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase,POD)活性无显著差异。解毒酶中,带毒烟粉虱羧酸酯酶(carboxylesterase,CarE)活性明显下降,是无毒烟粉虱活性的54%,谷胱甘肽S转移酶(glutathione-s-transferase,GST)和乙酰胆碱酯酶(acetylcholin esterase,AChE)活性无显著差异。To explicit the impact of Tomato chlorosis virus(ToCV)on main biological traits of its vector Bemisia tabaci,the biological parameters of B.tabaci Q infected by ToCV were detected on cotton(a non-host of the virus),and activities of main protective and detoxification enzymes were detected in B.tabaci Q virus infected and non-infected one.The results showed that the developmental period,fecundity and longevity were not different apparently between infected and non-infected B.tabaci.The body size of female adult of infected ones was shorter than non-infected ones.Compared to non-infected B.tabaci,activities of catalase(CAT)in infected B.tabaci increased to 3.36 times(P<0.001),while activity of superoxide dismutase(SOD)and peroxidase(POD)in infected B.tabaci didn't change significantly.In detoxification enzymes,carboxylesterase(CarE)activity in infected B.tabaci decreased significantly and which was only 54%of that of non-infected ones.Glutathione-s-transferase(GST)and acetylcholin esterase(AChE)showed no difference between infected and non-infected B.tabaci.

关 键 词：烟粉虱 番茄褪绿病毒 生物学 保护酶 解毒酶 

分 类 号：Q968.3[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]