Hepatoprotective ability of tetramethylpyrazine produced by Bacillus amyloliquefaciens  

作　　者：Wenqing Zhang Guanru Si Zhiming Rao Shuai Zong Jinglei Li Xian Zhang Chuanqiang Gao Zhou Ping Ming Ye 

机构地区：[1]School of Food and Biological Engineering,Hefei University of Technology,230009 Hefei,China [2]Research Institute of Jiangnan Small Pit Brewing Technology,Xuancheng 242000,China [3]The Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Biotechnology,Jiangnan University,Wuxi 214122,Jiangsu,China [4]College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,Jiangsu,China

出　　处：《Systems Microbiology and Biomanufacturing》2021年第2期223-233,共11页系统微生物学与生物制造（英文）

基　　金：This work was supported by the Xuanjiu Group Co.(W2016JSKF0081).

摘　　要：Tetramethylpyrazine(TTMP),an important aroma compound,was produced by Bacillus amyloliquefaciens XJB-104 with distillers’grains as raw material.The yield of TTMP under optimized fermentation procedure was 3176.52 mg/L.TTMP in the fermentation broth was purified and the purity(>99%)was measured by gas chromatography-mass spectrometry.Furthermore,the hepatoprotective activity of TTMP in ethanol-water system was evaluated by biochemical indicators of liver injury,parameters of antioxidant defense system and inflammatory response,and liver histopathological assessment in mice.Ethanol treatment increased serum levels of alanine aminotransferase,aspartate aminotransferase,alkaline phos-phatase and lactate dehydrogenase,suggesting liver damage.Both high and low dose of TTMP significantly decreased levels of these indicators.Furthermore,the reduction in the activities or concentrations of superoxide dismutase,catalase,reduced glutathione and malondialdehyde in liver tissue caused by ethanol was significantly alleviated by TTMP.Increased inflammatory cytokines including transcription factor,tumor necrosis factor,interleukin-1beta,interleukin,macrophage chemoattractant protein,inducible nitric oxide synthase and cyclooxygenase were also suppressed by TTMP treatment.It is concluded that TTMP in ethanol-water system has potential liver-protective activity,especially when ethanol is consumed at low doses for short periods of time.

关 键 词：TETRAMETHYLPYRAZINE Bacillus amyloliquefaciens Hepatoprotective effect Distillers’grains Ethanol-water system 

分 类 号：TQ9[化学工程]