一株低致病性H5N6亚型禽流感病毒(AIV)的生物学特性分析  

Biological characteristics of one low pathogenic H5N6 avian influenza virus

作　　者：李明慧 田井满 白晓利 刘丽娜[1] 李雁冰[1] 陈化兰[1] LI Ming-hui;TIAN Jing-man;BAI Xiao-li;LIU Li-na;LI Yan-bing;CHEN Hua-lan(Animal Influenza Key Laboratory of the Ministry of Agriculture and Rural Affairs,State Key Laboratory of veterinary Biotechnology,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China)

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/农业农村部动物流感重点开放实验室,黑龙江哈尔滨150069

出　　处：《中国预防兽医学报》2021年第8期801-805,811,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金创新群体项目(31521005)。

摘　　要：2019年11月本实验室从家禽监测样品中分离鉴定到一株低致病性H5N6亚型禽流感病毒(AIV),命名为A/chicken/Hebei/S1/2019(H5N6)(简称CK/HeB/S1/19)。为进一步了解其生物学特点,本实验对其全基因组测序并进行遗传演化分析,结果显示,该病毒属于2.3.4.4h分支,碱性裂解位点为PLRESR↓GLF,属于低致病性AIV(LPAIV)。对其各基因节段分别经BLAST比对后发现,其外部基因来源于H5N6亚型AIV,内部基因完全来源于H9N2亚型AIV,推测该分离株为一株重组病毒。将CK/HeB/S1/19病毒株接种SPF鸡及BALB/c小鼠研究其致病性,结果显示,该病毒虽然能够感染鸡,但不能在鸡体内有效复制,而该病毒未经适应就可在3只小鼠的鼻甲和肺中进行复制,对小鼠呈低致病性。本研究为H5亚型AIV的检测和其感染的综合防控提供了参考依据。One low pathogenic H5 N6 subtype avian influenza virus(AIV), A/chicken/Hebei/S1/2019(H5 N6)(CK/HeB/S1/19),was isolated from the collected poultry samples during surveillance. The whole genome of the isolate were sequenced, and the results showed that the virus belonged to clade 2.3.4.4 h and the polybasic motif at the HA cleavage site of the virus was PLRESR↓GLF, which meets the criterion for low pathogenic AIVs. The BLAST analysis of each fragment revealed that the isolate was a natural reassortant AIV, which bears its surface genes from H5 N6 viruses and the whole internal genes from H9 N2 viruses. SPF chickens and BALB/c mice were infected with this isolate to evaluate its pathogenicity. Animal experiments indicated that the virus infection could be observed in chickens but the virus could not replicate in any organs of the inoculated chickens, and the virus could replicate in lungs and the nasal turbinates of infected mice without preadaptation but showed low pathogenicity to mice. The finding will facilitate the surveillance and the infection control of H5 subtype influenza viruses.

关 键 词：禽流感病毒 低致病性 H5N6 进化分析 致病性 

分 类 号：S852.65[农业科学—基础兽医学]