本源菌降解褐煤的生物学特性及微观结构分析  

Research on biological characteristics and microstructure of lignite degradation by indigenous microorganism

作　　者：牛显 牛煜 索永录[1] NIU Xian;NIU Yu;SUO Yonglu(College of Energy Science and Engineering,Xi’an University of Science and Technology,Xi’an 710054,China;School of Mining and Technology,Inner Mongolia University of Technology,Hohhot 010051,China;School of Electric Power,Civil Engineering and Architecture,Shanxi University,Taiyuan 030006,China)

机构地区：[1]西安科技大学能源学院,陕西西安710054 [2]内蒙古工业大学矿业学院,内蒙古呼和浩特010051 [3]山西大学电力与建筑学院,山西太原030006

出　　处：《西安科技大学学报》2021年第5期836-844,共9页Journal of Xi’an University of Science and Technology

基　　金：内蒙古自治区自然科学基金项目(2020MS05025);内蒙古工业大学科学研究项目(ZY201823)。

摘　　要：从原位褐煤中筛选本源菌并分析其降解特性是探索原位微生物降解转化煤炭的有效途径。经过原位取样,富集培养,纯化分离得到一株本源真菌,以形态学观测和ITS测序,确定为镰刀菌属(F.spp.NF01)。通过本源菌F.spp.NF01生长曲线、pH-t曲线及褐煤的降(溶)解实验研究了其生理学特性,并利用扫描电子显微镜、傅里叶红外光谱、电子顺磁共振分析了残煤表面形貌、官能团及自由基。结果表明:在MM-SGC培养基中,本源菌F.spp.NF01生长液呈现碱性,菌液含煤时pH值达9.41,0.5 g褐煤可被生长6 d的气生菌丝溶解,且褐煤的降(溶)解率随pH值升高而增大;本源菌F.spp.NF01作用后的残煤,表面离散颗粒更小,粗糙和裂隙度更大,芳环侧链基团、醚键、芳香族■、脂肪族-CH2-和-CH3-吸收峰强度减弱,自由基浓度Ng、朗德因子g值和线宽ΔH明显增大。说明本源菌F.spp.NF01可以降(溶)解褐煤,使其形貌和结构改变,产生的碱性物质参与了褐煤的降(溶)解。该研究为本源菌降解褐煤的微观结构研究起到积极地促进作用。Screening indigenous microorganism to explore lignite degrading capacity is an efficient way to examine in-situ biotransformation of lignite.An indigenous fungus was obtained by means of in-situ sampling, enrichment culture, purification and isolation, which was identified as Fusarium(F.spp.NF01)from morphological observation and ITS sequencing.The biological characteristics of indigenous F.spp.NF01 were studied based on strain growth curves, pH-t curves and degradation/dissolution experiment of lignite, and scanning electron microscopy, fourier transform infrared spectroscopy and electron paramagnetic resonance were used to analyze the surface morphology, functional groups and free radicals of the residual coal.The results showed that in MM-SGC medium, the growth solution of indigenous F.spp.NF01 was alkaline, and the pH value was up to 9.41 with the growth liquid containing lignite.0.5 g lignite was dissolved by aerial hyphae grown for 6 days, and the degradation/dissolution rate of lignite promoted with the increase of pH value.With indigenous F.spp.NF01 acted on the pretreatment lignite sample, the surface discrete particles of the residual coal became smaller, accompanied by greater roughness and crack structures, the intensity of the aromatic ring side chain groups, ether bonds, aromatic ■,aliphatic -CH2- and -CH3- absorption peak was weakened, and the concentration of free radical Ng, Landé g-factor and line width ΔH were significantly increased.This indicates that indigenous F.spp.NF01 can degrade/dissolve the lignite to change its morphology and structure.Alkaline substances secreted by F.spp.NF01 are involved in lignite degradation/dissolution.The research will provide a guidance for the study of the microstructure of lignite degradation by indigenous microorganism.

关 键 词：褐煤 生物降解 表面形貌 官能团 自由基 本源菌 镰刀菌 

分 类 号：TD989[矿业工程—选矿]