扩张型心肌病大鼠心肌细胞亚细胞器超微结构分析  

Ultrastructure of sub-organelles of cardiomyocytes in rats with dilated cardiomyopathy

作　　者：常红波[1] 王振涛[2] 吴鸿[1] CHANG Hongbo;WANG Zhentao;WU Hong(Laboratory of Cell Imaging,Henan University of Chinese Medicine,Zhengzhou 450002;Department of Cardiovascular,Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou 450002,China)

机构地区：[1]河南中医药大学细胞成像实验室,郑州450046 [2]河南省中医院心血管科,郑州450002

出　　处：《国际心血管病杂志》2021年第5期292-295,共4页International Journal of Cardiovascular Disease

基　　金：河南中医药大学博士科研启动基金(DSRSBSJJ2019-31);河南省特色骨干学科中医学学科建设项目(STG-ZYXKY-2020035)。

摘　　要：目的:观察扩张型心肌病(DCM)大鼠心肌细胞亚细胞器超微结构变化。方法:将60只Wistar大鼠随机分为正常组(n=10)、DCM组(n=50)。正常组大鼠给予基础饮食,未进行干预;DCM组大鼠每日给予70%呋喃唑酮溶液诱导DCM模型,给药10周。采用超声心动仪检测大鼠心脏功能,透射电子显微镜观察各组大鼠心肌细胞亚细胞器变化。结果:与正常组比较,DCM组左室收缩末期内径(LVESD)和左室舒张末期内径(LVEDD)增加,左室缩短分数(LVFS)和左室射血分数(LVEF)降低(P均<0.05)。与正常组比较,DCM组心肌细胞核增大,线粒体出现肿胀、空泡化,闰盘中间连接和桥粒结构模糊,内质网紊乱,溶酶体异常增大且附近散在糖原颗粒,横管结构破坏,肌原纤维排列紊乱。结论:DCM大鼠存在心肌细胞亚细胞器损伤及细胞器间联系失衡。Objective:To analyze the ultrastructural changes of sub-organelles of cardiomyocytes in rats with dilated cardiomyopathy(DCM).Methods:Sixty Wistar rats were randomly divided into normal group(n=10)and DCM group(n=50).The rats in normal group were fed with basic diet only,while the rats in DCM group were given 70%furazolidone solution daily for 10 weeks to establish DCM models.The function of heart were quantified by echocardiography.Transmission electron microscopy was used to observe the ultrastructural changes of sub-organelles of cardiomyocytes in both groups.Results:Compared with the normal group,left ventricular end systolic diameter(LVESD)and left ventricular end diastolic diameter(LVEDD)in model group were higher,while left ventricular fractional shortening(LVFS)and left ventricular ejection fraction(LVEF)were lower(both P<0.05).Compared with the normal group,the nucleus of the model group was enlarged,and the mitochondria appeared swollen and vacuolated.The structure of the middle junction and desmosome in the disc were blurred,with disordered endoplasmic reticula.The lysosome was abnormally enlarged and there were scattered glycogen particles nearby.The structure of transverse canal was destroyed,and the myofibrils were disordered.Conclusions:The damage of sub-organelles and imbalance of organelle exist in cardiomyocytes of rats with DCM.

关 键 词：扩张型心肌病 心肌细胞亚细胞器 超微结构 透射电子显微镜 

分 类 号：R542.2[医药卫生—心血管疾病]