Characterization,application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9  

作　　者：Mayur Gahlout Poonam B.Chauhan Hiren Prajapati Nirali Tandel Seema Rana Dhrutikumari Solanki Nilamben Patel 

机构地区：[1]Department of Microbiology,KBS Commerce and NATARAJ Professional Sciences College(Affiliated To VNSGU,Surat),Vapi 396195,India

出　　处：《Systems Microbiology and Biomanufacturing》2021年第4期459-470,共12页系统微生物学与生物制造（英文）

摘　　要：The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9.The work involves optimiza-tion of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition.During optimization process,maximum pyocyanin production(92.12μg/ml)was obtained with carbon source mannitole,nitrogen source peptone,inorganic salt NaCl and metal ion FeSO_(4).Plackett Burman design and Response Surface Methodology(RSM)showed peptone,NaCl and KH_(2)PO_(4)are significant variable in the production of pyocyanin pigment.The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment.The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms.Thus,present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.

关 键 词：Pseudomonas aeruginosa DN9 PYOCYANIN Plackett Burman design Response surface methodology FTIR 

分 类 号：F42[经济管理—产业经济]