玉郎伞查耳酮下调Cav1.2和RyR2表达抑制氧化应激所致心肌钙超载的效果及作用机制  

Inhibitory effect and action mechanism of Yulangsan chalcone on oxidative stress-induced myocardial calcium overload through down-regulating Cav1.2 and RyR2 expression

作　　者：蒙湄方 苏延旭[2] 马兴菊 何阳 黄仁彬[1] 李映新[1] MENG Mei-fang;SU Yan-xu;MA Xing-ju;HE Yang;HUANG Ren-bin;LI Ying-xin(School of Pharmacy,Guangxi Medical University,Nanning 530021,China;Department of Pharmacy,the Second Affiliated Hospital of Guangxi Medical University,Nanning 530007,China;Department of Pharmacy,Affiliated Ruikang Hospital of Guangxi University of Chinese Medicine,Nanning 530011,China)

机构地区：[1]广西医科大学药学院,南宁市530021 [2]广西医科大学第二附属医院药剂科,南宁市530007 [3]广西中医药大学附属瑞康医院药剂科,南宁市530011

出　　处：《广西医学》2021年第14期1708-1711,共4页Guangxi Medical Journal

基　　金：广西一流学科(药学)建设项目(GXFCDP-PS-2018)。

摘　　要：目的分析玉郎伞查耳酮(YLSC)预处理对氧化应激所致心肌钙超载的干预效果及作用机制。方法取乳鼠心尖组织用于培养心肌细胞。将原代培养的心肌细胞分成对照组、模型组、100μmol/L YLSC预处理组和200μmol/L YLSC预处理组。预处理组用含相应终浓度的YLSC的无血清培养基孵育,其他两组加入无血清培养基孵育。24 h后,除对照组外,其他组加入过氧化氢(0.3 mmol/L)诱导氧化应激,立刻以Fluo-3AM为荧光探针,激光共聚焦显微镜动态检测细胞内游离钙离子浓度;诱导15 min后,检测钙离子通道Cav1.2以及雷诺定受体2(RyR2)的mRNA表达情况。结果模型组、各剂量YLSC预处理组的游离钙离子浓度均高于对照组,而模型组、100μmol/L YLSC预处理组和200μmol/L YLSC预处理组的游离钙离子浓度依次降低(均P<0.05)。模型组Cav1.2和RyR2的mRNA表达水平高于对照组(P<0.01),而模型组、100μmol/L YLSC预处理组、200μmol/L YLSC预处理组的Cav1.2和RyR2 mRNA表达水平依次降低(均P<0.05)。结论YLSC预处理可抑制氧化应激引起的心肌钙超载,其可能通过减少Cav1.2、RyR2的基因表达发挥作用。Objective To analyze the intervention effect and action mechanism of Yulangsan chalcone(YLSC)preconditioning on oxidative stress-induced myocardial calcium overload.Methods Apex tissues of neonatal rats were collected for cardiomyocyte culture.Primary cultured cardiomyocytes were divided into control group,model group,100μmol/L and 200μmol/L YLSC preconditioning groups.The preconditioning groups were incubated using serum-free medium containing YLSC with corresponding final concentration,and the remaining two groups were incubated using serum-free medium alone.After 24 hours,except for the control group,oxidative stress induction was conducted on the other groups by hydrogen peroxide(0.3 mmol/L),and laser confocal microscopy was used to dynamically detect the free calcium[Ca 2+]i concentration immediately with Fluo-3AM as fluorescence probe.After 15 minutes of induction,the mRNA expression of Cav1.2 calcium channel and ryanodine receptor 2(RyR2)was determined.Results The concentration of[Ca 2+]i was higher in the model group and all YLSC preconditioning groups than in the control group,and decreased in the order of the model group,the 100μmol/L YLSC preconditioning group and the 200μmol/L YLSC preconditioning group(all P<0.05).The expression levels of Cav1.2 and RyR2 mRNAs were higher in the model group than in the control group(P<0.01),and decreased in the model group,100μmol/L and 200μmol/L YLSC preconditioning groups successively(all P<0.05).Conclusion YLSC preconditioning can inhibit oxidative stress-induced myocardial calcium overload,and it may exert the effect through suppressing the mRNA expression of Cav1.2 and RyR2.

关 键 词：玉郎伞查耳酮 心肌细胞 氧化应激 钙超载 过氧化氢 

分 类 号：R285.5[医药卫生—中药学]