HPLC法同时测定感冒疏风丸中蛇床子素和二氢欧山芹醇当归酸酯的含量  

Simultaneous Determination of Osthol and Dihydrooxanol Angelica Acid Ester in Ganmao Shufeng Pills by HPLC

作　　者：杨深应 刘常逊 上官云兰 陶明宝 柴连周[2] 赵兴蕊 YANG Shen-ying;LIU Chang-xun;SHANG-guan Yun-lan;TAO Ming-bao;CHAI Lian-zhou;ZHAO Xing-rui(Food and Drug Inspection and Testing Center of Baoshan City,Baoshan 678000,China;Baoshan Junior College of Traditional Chinese Medicine,Baoshan 678000,China;School of Pharmacy,Dali University,Dali 671000,China)

机构地区：[1]保山市食品药品检验检测中心,云南保山678000 [2]保山中医药高等专科学校,云南保山678000 [3]大理大学药学院,云南大理671000

出　　处：《云南中医中药杂志》2021年第9期72-75,共4页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：云南省教育厅科学研究基金项目(2021J1133);保山市科技计划项目(2020ZC002);保山中医药高等专科学校百名中青年学术技术带头人(2020x001)。

摘　　要：目的建立高效液相色谱法(HPLC)同时测定感冒疏风丸中蛇床子素和二氢欧山芹醇当归酸酯含量的方法。方法采用Agilent 5TC-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(49∶51),流速1.0 mL/min,检测波长330 nm,柱温30.0℃。结果蛇床子素和二氢欧山芹醇当归酸酯线性范围分别为0.0451~1.3533μg(r=0.9999)和0.0164~0.4911μg(r=0.9999);平均回收率分别为98.74%和97.09%,RSD分别为0.35%和0.29%。结论该方法专属性强、结果准确、操作简便,可用于感冒疏风丸的质量控制。Objective:To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of osthol and dihydrooxanol angelica acid ester in Ganmao Shufeng Pills.Methods:An agilent 5TC-C18 column(4.6 mm×250 mm,5μm)was used,the mobile phase was acetonitrile-water(49:51),the flow rate was 1.0 mL/min,the detection wavelength was 330 nm,and the column temperature was 30.0℃.Results:The linear ranges of osthole and dihydrooxanol angelica acid este were 0.0451~1.3533μg(r=0.9999)and 0.0164~0.4911μg(r=0.9999),respectively and the average recoveries were 98.74%and 97.09%respectively,and the RSDs were0.35%and 0.29%.It is 0.35%and 0.29%respectively.Conclusion:The method has strong specificity,accurate results and simple operation,and can be used for the quality control of Ganmao Shufeng Pills.

关 键 词：感冒疏风丸 高效液相色谱法 蛇床子素 二氢欧山芹醇当归酸酯 含量测定 

分 类 号：R285.5[医药卫生—中药学]