化瘀通络汤对局灶性脑缺血-再灌注损伤大鼠的脑神经保护作用  

Neuroprotective Effect of Stasis-Activating and Collaterals-Dredging Decoction on Focal Cerebral Ischemia-Reperfusion Injury in Rats

作　　者：彭慧渊[1] 胡莹[1] 杨楠[1] 王本国[1] 王秉道 汪峰[1] 林铎 PENG Hui-Yuan;HU Ying;YANG Nan;WANG Ben-Guo;WANG Bing-Dao;WANG Feng;LIN Duo(Zhongshan Hospital of Chinese Medicine,Zhongshan 528400 Guangdong,China)

机构地区：[1]广东省中山市中医院,广东中山528400

出　　处：《广州中医药大学学报》2021年第9期1941-1948,共8页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：中山市科技计划医疗卫生重大专项(编号:2014A1FC007);国家中医药管理局中医临床研究基地科研立项(编号:JDZX2015296)。

摘　　要：【目的】探讨化瘀通络汤对局灶性脑缺血-再灌注损伤大鼠的脑神经保护作用。【方法】将100只SD大鼠随机分为假手术组(蒸馏水灌胃),模型组(蒸馏水灌胃),化瘀通络汤低、中、高剂量组(7.65、15.30、30.60 g/kg对应灌胃),脑心通组(步长脑心通溶液0.864 g/kg灌胃)。各组大鼠连续灌胃7 d后,除假手术组,其余各组大鼠采用改良线栓法制备大脑中动脉栓塞模型。于造模后24 h采用改良神经功能缺损评分(mNSS)评估神经功能。处死大鼠后,采用2,3,5-氯化三苯基四氮唑(TTC)染色法测定脑梗死范围,苏木素-伊红(HE)染色法观察大鼠脑组织病理损伤变化,甲苯胺蓝染色法观察大鼠脑组织神经细胞形态及受损情况,原位末端转移酶标记(TUNEL)染色法观察大鼠神经元细胞凋亡情况,免疫组织化学法观察大鼠脑组织碱性成纤维细胞生长因子(bFGF)蛋白表达,酶联免疫吸附分析(ELISA)法检测大鼠血清炎性因子白细胞介素6(IL-6)表达水平。【结果】与假手术组比较,模型组大鼠神经功能缺损评分升高(P<0.05),脑梗死体积增大(P<0.05),大脑组织出现明显病理损伤,神经细胞凋亡率增加(P<0.05),梗死区域b FGF蛋白表达水平降低(P<0.05),血清中IL-6表达水平升高(P<0.05);与模型组比较,化瘀通络汤各剂量组上述指标得到明显改善,效果与脑心通组相当。【结论】化瘀通络汤对局灶性脑缺血-再灌注损伤大鼠有神经保护作用。Objective To explore the neuroprotective effect of Stasis-Activating and Collaterals-Dredging Decoction(SCD)on the focal cerebral ischemia-reperfusion injury in rats. Methods One-hundred rats were randomly divided into sham-operation group(distilled water by gavage),model group(distilled water by gavage),low-,middle-and high-dose SCD groups(7.65,15.30,30.60 g·kg^(-1)·d^(-1) of SCD by gavage,respectively),Naoxintong group(0.864 g·kg^(-1)·d^(-1) of Buchang Naoxintong solution by gavage). After intragastric administration for 7 continuous days, the rat model of middle cerebral artery occlusion(MCAO)was established by modified thread-occlusion method. Twenty-four hours after modeling,the neurological function was assessed by modified neurological severity score(mNSS). After cervical luxation of rats,the infarct area of brain was measured by 2,3,5-triphenyltetrazolium chloride(TTC) staining, the pathological changes of brain tissue were observed by hematoxylin-eosin(HE)staining,the morphological characteristics of nerve cells were observed by toluidine blue staining,the cell apoptosis was observed by terminal deoxynucleotidyl transferase(TdT)-mediated dUTP-biotin nick end labeling(TUNEL)staining,the protein expression level of basic fibroblast growth factor(bFGF)in brain tissue was detected by immunohistochemistry,and the level of inflammatory factor interleukin 6(IL-6)in serum was detected by enzyme-linked immune-sorbent assay(ELISA). Results Compared with the sham-operation group,the mNSS was increased in the model group(P< 0.05),and the cerebral infarction volume was enhanced(P< 0.05),the obvious pathological injury in rat brain tissue was seen,the rate of neuronal apoptosis was raised(P< 0.05),the expression level of bFGF in the infarct area was lowered(P< 0.05),and the level of IL-6 in serum was increased(P< 0.05). Compared with the model group,the above indexes were improve in the various dose SCD groups, the effect being equivalent to that of the Naoxintong group. Conclusion SCD has neuroprotective effects

关 键 词：化瘀通络汤 局灶性脑缺血-再灌注损伤 神经保护 大鼠 

分 类 号：R285.5[医药卫生—中药学]