坤宝丸对大鼠实验性卵巢早衰模型的影响  

Effects of Kunbao Pills on Premature Ovarian Failure in Rats

作　　者：李想[1] 李嘉[1] LI Xiang;LI Jia(Cancer Hospital of China Medical University,Liaoning Cancer Hospital,Shenyang 110042 Liaoning,China)

机构地区：[1]中国医科大学肿瘤医院、辽宁省肿瘤医院,辽宁沈阳110042

出　　处：《广州中医药大学学报》2021年第9期1949-1954,共6页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：【目的】探讨坤宝丸对实验性卵巢早衰大鼠模型的防治作用及机制。【方法】将60只大鼠随机分为空白组、模型组、坤宝丸低剂量组(0.9 g·kg^(-1)·d^(-1))、坤宝丸中剂量组(1.8 g·kg^(-1)·d^(-1))、坤宝丸高剂量组(3.6 g·kg^(-1)·d^(-1))、结合雌激素组(0.12 g·kg^(-1)·d^(-1)),每组10只。预防给药14 d后,除空白组,其他各组大鼠分别于第14、21天腹腔注射顺铂(4 mg/kg)建立卵巢早衰模型,持续灌胃给药至实验的第28天。实验期间采用阴道涂片观察大鼠动情周期的变化。给药结束后,检测大鼠血清促卵泡激素(FSH)、血清雌二醇(E2)、血清促黄体生成激素(LH)水平,免疫组织化学法检测大鼠卵巢组织转化生长因子(TGF)-β1、结缔组织生长因子(CTGF)及干细胞因子(SCF)的表达。【结果】与空白组比较,模型组大鼠的动情周期均有所延长,血清FSH、LH,卵巢组织CTGF、TGF-β1、SCF水平均升高(P<0.01),血清E2水平降低(P<0.01)。与模型组比较,坤宝丸低剂量组大鼠大部分出现动情周期紊乱,坤宝丸中、高剂量组及结合雌激素组大鼠多恢复正常动情周期;与模型组比较,坤宝丸低、中、高剂量组及结合雌激素组血清FSH、LH,卵巢组织TGF-β1水平显著降低(P<0.05或P<0.01),坤宝丸中、高剂量组及结合雌激素组血清E2水平显著升高,SCF表达水平显著降低(P<0.05或P<0.01),坤宝丸高剂量组及结合雌激素组卵巢组织CTGF表达显著降低(P<0.01)。各治疗组间上述指标比较,差异均无统计学意义(P> 0.05)。【结论】坤宝丸对顺铂诱导的大鼠卵巢早衰模型具有积极的干预作用,其机制可能与减轻卵巢纤维化有关。Objective To explore the preventative and therapeutic effect of Kunbao Pills for premature ovarian failure in rats. Methods Sixty rats were randomly divided into blank group,model group,low-dose Kunbao Pills group(0.9 g · kg-1· d-1), middle-dose Kunbao Pills group(1.8 g · kg-1· d-1), high-dose Kunbao Pills group(3.6 g·kg^(-1)·d^(-1)),and estrogen combination group(0.12 g·kg^(-1)·d^(-1)),10 rats in each group. After 14 days of prophylactic administration,a rat model of premature ovarian failure in various groups apart from the blank group was established by intraperitoneal administration of cisplatin(4 mg/kg) on experimental day 14 and 21,respectively,and the intragastric administration continued until experimental day 28. During the modeling,the change of rat estrous cycle was observed by vaginal smear. After medication ending, serum levels of folliclestimulating hormone(FSH),estradiol(E2)and luteinizing hormone(LH)were determined,and expression levels of transformed growth factor(TGF)-β1,connective tissue growth factor(CTGF)and stem cell factor(SCF)in ovarian tissue were detected by immunohistochemical staining. Results Compared with the blank group, the prolonged estrous cycle was showed in the model group,the levels of FSH,LH in serum and CTGF,TGF-β1,SCF in ovarian tissue were increased(P< 0.01),level of E2 in serum was decreased(P< 0.01). Compared with the model group,the disordered estrous cycle was seen in most rats of the low-dose Kunbao Pills group,while the disordered estrous cycle was recovered to normal level in most rats of the middle-and high-dose Kunbao Pills groups and estrogen combination group. Compared with the model group,the levels of FSH and LH in serum and TGF-β1 in ovarian tissue in the low-,middle-and high-dose Kunbao Pills groups and estrogen combination group were decreased significantly(P< 0.05 or P< 0.01). the levels of E2 in serum and SCF in ovarian tissue in the middle-and high-dose Kunbao Pills groups and estrogen combination group were significantly increased(P< 0.05 or

关 键 词：坤宝丸 卵巢早衰 转化生长因子Β1 结缔组织生长因子 干细胞因子 卵巢纤维化 大鼠 

分 类 号：R285.5[医药卫生—中药学]