Genome sizes of four important medicinal species in Kadsura by flow cytometry  

作　　者：Jing Xu Xue-ping Wei Jiu-shi Liu Yao-dong Qi Ben-gang Zhang Hai-tao Liu Pei-gen Xiao 

机构地区：[1]Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China [2]Engineering Research Center of Tradition Chinese Medicine Resource,Ministry of Education,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China

出　　处：《Chinese Herbal Medicines》2021年第3期416-420,共5页中草药（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Grant No.81872965,81703650)。

摘　　要：Objective:Dianjixueteng is a geoherb in Yunnan Province,the source plant of which is Kadsura interior.However,the formation of this geoherb is not clear in genetic mechanism,in which genome size is the first step that should be known on the genomic level.In this study we aimed to estimate the genome sizes of source plants of K.interior and three related herbs K.heteroclita,K.longipedunculata,and K.coccinea by flow cytometry(FCM)and make a comparison.Methods:The genome sizes of K.interior,K.heteroclita,K.longipedunculata and K.coccinea,i.e.,the source plants of Dianjixueteng and its relative medicinal materials,were estimated by FCM.The nuclei of K.interior were isolated using modified LB01 buffer,for the rest species,by the Galbraith’s buffer.Results:The genome sizes of K.interior,K.heteroclita,K.longipedunculata,and K.coccinea were 7.36,7.12,7.01,and 5.15 pg/1 C,respectively.Genome size of K.interior had no significant variation with those of K.heteroclita and K.longipedunculata(P=0.296),which was significantly larger than that of K.coccinea.Conclusion:Genome size can not distinguish K.interior from K.heteroclita and K.longipedunculata,but could distinguish them from K.coccinea,which lays the foundation for future studies on genetic mechanism of the geoherb formation.

关 键 词：Dianjixueteng Flow cytometry Genome size Kadsura interior A.C.Smith 

分 类 号：S567[农业科学—中草药栽培]