盐胁迫对草莓苗期生长及氧化还原系统的影响  

Effects of Salt Stress on Strawberry Seedling Growth and Antioxidant System

作　　者：姚玉涛[1] 张国新[1] 丁守鹏 孙叶烁 YAO Yutao;ZHANG Guoxin;DING Shoupeng;SUN Yeshuo(Coastal Agriculture Institute,Hebei Academy of Agriculture and Forestry Sciences/Hebei Technical Research Center of Saline-alkali Soil Greening Project/Tangshan Key Laboratory of Plant Salt Tolerance Research,Caofeidian,Hebei 063200)

机构地区：[1]河北省农林科学院滨海农业研究所,河北省盐碱地绿化工程技术研究中心,唐山市植物耐盐研究重点实验室,河北曹妃甸063200

出　　处：《北方园艺》2021年第17期22-29,共8页Northern Horticulture

基　　金：河北省创新工程资助项目(2019-1-6-4);唐山市科技计划资助项目(19150255E)。

摘　　要：以12个国内市场主栽草莓品种为试材,采用微咸水灌溉的方法,研究了不同草莓品种的叶柄长、平均茎粗、SPAD、根长、K+/Na+、根冠比、丙二醛(MDA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及盐害指数等指标变化情况,以期为草莓苗期耐盐性评价提供参考依据。结果表明:在T1(3 g·L^(-1))处理条件下,9号("冀纯")、11号("白雪公主")、12号("章姬")草莓品种盐害指数分别为19.58%、13.92%和18.68%,均低于20%,属于高耐性草莓品种范畴;淡水处理条件下,12个草莓品种叶片MDA含量较低,T1处理12个草莓品种叶片MDA含量较CK处理均显著增加,其中9、11号和12号3个草莓品种较对照增加值最低,说明细胞膜受到的膜脂氧化伤害最低;随着微咸水灌溉浓度的增加,12个草莓品种叶片中的SOD、POD、CAT活性均显著降低。通过盐害指数法、草莓叶片MDA含量及相关抗氧化酶活性的变化情况3种研究方法,综合分析得出3个适宜微咸水灌溉的高耐盐性草莓品种9号("冀纯")、11号("白雪公主")、12号("章姬")。Taking 12 domestic market main strawberry varieties as the test materials, the changes of different strawberry varieties were studied by stalk length, average stem thickness, SPAD,root length, K+/Na+,root crown ratio, MDA,POD,SOD,CAT and salt damage index, in order to provide a reference for the evaluation of salt tolerance in strawberry seedling stage.The results showed that under T1(3 g·L^(-1)) treatment conditions, the salt damage indexes of strawberry varieties No.9(‘Ji Chun’),No.11(‘Bai Xue Princess’),and No.12(‘Zhang Ji’) were 19.58%,13.92% and 18.68%,respectively.The content of MDA in the leaves of 12 strawberry varieties was lower under freshwater treatment conditions, and the MDA content in the leaves of 12 strawberry varieties in T1 treatment was significantly higher than that in CK treatment.Among them, No.9 and No.11 compared with the control, the three strawberry varieties and No.12 have the lowest increase in value, indicating that the membrane lipid oxidation damage to the cell membrane was the lowest;with the increase in the concentration of brackish water irrigation, the SOD,POD and CAT enzyme activities in the leaves of the 12 strawberry varieties were significantly reduced.Through the three research methods of salt damage index method and the changes of strawberry leaf MDA content and related antioxidant enzyme activities, a comprehensive analysis has obtained three high salt-tolerant strawberry varieties No.9(‘Jichun’),No.11(‘Snow White’) and No.12(‘Zhang Ji’) that are suitable for brackish water irrigation.

关 键 词：草莓苗期 耐盐性 盐害指数 MDA 抗氧化酶活性 

分 类 号：S668.4[农业科学—果树学]