商丘地区草莓叶斑病的分离与鉴定  

Isolation and Identification of Strawberry Leaf Spot Disease in Shangqiu

作　　者：朱晓琴[1] 张奇 郑爱珍[1] 韩霜[1] 裴冬丽[1] ZHU Xiaoqin;ZHANG Qi;ZHENG Aizhen;HAN Shuang;PEI Dongli(Henan Provincial Key University Laboratory for Plant-microbe Interactions,Shangqiu Normal University,Shangqiu,Henan 476000)

机构地区：[1]商丘师范学院河南省高校植物与微生物互作重点实验室,河南商丘476000

出　　处：《北方园艺》2021年第17期38-43,共6页Northern Horticulture

基　　金：国家自然科学基金资助项目(31902066);河南省自然科学基金资助项目(182300410058);河南省高校科技创新团队(21IRTSTHN025)。

摘　　要：以商丘双八镇草莓为试材,采用组织分离法,研究了引起草莓叶斑病的病原菌鉴定,以期为草莓种植过程中叶斑病防治提供参考依据。结果表明:引起草莓叶斑病的病原菌是一株真菌,编号为SQZQ-06,通过柯赫法则对该菌株的致病性进行测定,发现该菌株可以导致草莓叶片出现斑状病害特征。PCR扩增SQZQ-06菌株18S rRNA基因得到长度为589 bp的序列片段,将测定结果在NCBI网站进行比对并作系统进化树分析,综合该真菌的形态学特征后确定SQZQ-06为斑污拟盘多毛孢Pestalotiopsis maculans。Taking the leaves of strawberry in Shuangba town, Shangqiu as material, the pathogen of strawberry leaf spot disease by tissue isolation was investigated, in order to provide a reference for the control of strawberry leaf spot disease.The results showed that a fungus SQZQ-06 from strawberry leaf spot in Shuangba town, Shangqiu, was identified as Pestalotiopsis maculans.The pathogenicity of the strain was determined by Coch rule, and it was found that the strain could lead to the necrosis of the strawberry leaves.The 18 S rRNA gene of SQZQ-06 was amplified by PCR for molecular identification, and the length was 589 bp.Blast result in NCBI and phylogenetic tree analysis suggested that SQZQ-06 was Pestalotiopsis maculans.According to the morphological characteristics and molecular identification of the fungus, SQZQ-06 was identified as Pestalotiopsis maculans.

关 键 词：草莓 叶斑病 形态特征 分子鉴定 

分 类 号：S668.4[农业科学—果树学]