苜蓿皂苷对X射线照射小鼠造血系统的影响  

Effect of alfalfa saponins on hematopoietic system of mice irradiated by X-ray

作　　者：张明[1,2] 李志威 刘建宇 张靖雪 郭志鹏 曲根[1] 严学兵 郭玉霞 ZHANG Ming;LI Zhiwei;LIU Jianyu;ZHANG Jingxue;GUO Zhipeng;Qu Gen;YAN Xuebing;GUO Yuxia(College of Animal and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China;Puyang Vocational and Technical College,Puyang 457000,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]濮阳职业技术学院,河南濮阳457000 [3]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《中国农业大学学报》2021年第7期78-84,共7页Journal of China Agricultural University

基　　金：国家“十三五”重点研发计划项目(2017YFD0502104)。

摘　　要：为研究苜蓿皂苷(Alfalfa saponin)对X射线照射小鼠造血系统的影响,选取雄性SPF级昆明系小鼠90只,随机分为对照组和试验组,研究25、50和100mg/ml的苜蓿皂苷对X射线照射强度为4.0Gy小鼠造血系统的影响和不同剂量X射线对添加25mg/ml的小鼠造血系统的影响。结果表明:1)预防性给予25mg/mL苜蓿皂苷,可显著减轻4.0Gy的X射线对小鼠外周血白细胞(White blood cells,WBC)、红细胞(Red blood cells,RBC)和血小板(Platelets,PLT)数量的降低程度(P<0.05),增加照射小鼠的骨髓有核细胞(Bone marrow nucleated cells,BMC)数(P<0.05),显著降低微核率(P<0.05)。2)预防性给予25mg/mL的苜蓿皂苷,相同照射剂量的试验组小鼠白细胞、红细胞、血小板和骨髓有核细胞数显著高于对照组(P<0.05),微核率显著低于对照组(P<0.05)。3)随照射强度增加,25mg/ml的苜蓿皂苷保护小鼠受辐射损伤的能力下降。综上,苜蓿皂苷可缓解X射线对小鼠造血系统和染色体的损伤,苜蓿皂苷浓度与射线强度存在量效关系,需要根据不同的照射强度添加不同浓度的苜蓿皂苷。In order to study the effect of alfalfa saponin on the hematopoietic system of X-ray irradiated mice,90 male SPF Kunming rice were selected and randomly divided into control group and experimental group.The effects of 25,50 and 100 mg/mL alfalfa saponin on the hematopoietic system of mice with X-ray irradiation intensity of 4.0 Gy and the effects of different doses of X-ray on the hematopoietic system of mice with X-ray irradiation intensity of 25 mg/mL of were studied.The results showed that:1)Preventive usuage of 25 mg/mL alfalfa saponin could significantly reduce the decrease of white blood cells(WBC),red blood cells(RBC)and platelets(PLT)in peripheral blood of mice exposed to 4.0 Gy X-ray(P<0.05),and increased the bone marrow nucleated cells(BMN)in irradiated mice.The number of cells(BMC)decreased significantly(P<0.05).2)After prophylactic administration of 25 mg/mL alfalfa saponin,the number of white blood cells,red blood cells,platelets and bone marrow nucleated cells in experimental group with the same irradiation dose was significantly higher than that in control group(P <0.05),and the micronucleus rate was significantly lower than that in control group(P<0.05).3)With the increase of radiation intensity,the protection ability of 25 mg/mL alfalfa saponin against radiation injury in mice decreased.To sum up,alfalfa saponin could alleviate the damage of hematopoietic system and chromosome of mice caused by X-ray.There was a dose-effect correlation between the concentration of alfalfa saponin and the radiation intensity of X-ray.Different concentration of alfalfa saponin should be applied according to different X-ray radiation intensity.

关 键 词：苜蓿皂苷 X射线 小鼠 造血系统 

分 类 号：R915[医药卫生—微生物与生化药学]