加减益经汤对卵巢功能减退大鼠转化生长因子β超家族相关成员的影响  

Effects of Modified Yijing Decoction on Transforming Growth FactorβSuperfamily-Related Members in Rats with Diminished Ovarian Reserve

作　　者：李丽美[1] 夏建红[1] 黄雪萍 陈婷婷[2] 和秀魁[3] LI Li-Mei;XIA Jian-Hong;HUANG Xue-Ping;CHEN Ting-Ting;HE Xiu-Kui(Dept.of Women Health Care,Guangdong Maternity and Child Care Hospital,Guangzhou 510095 Guangdong,China;Dept.of Health Care,Guangdong Maternity and Child Care Hospital,Guangzhou 510095 Guangdong,China;Dept.of Gynecology,Guangdong Maternity and Child Care Hospital,Guangzhou 510095 Guangdong,China)

机构地区：[1]广东省妇幼保健院妇保科,广东广州510095 [2]广东省妇幼保健院保健部,广东广州510095 [3]广东省妇幼保健院妇科,广东广州510095

出　　处：《广州中医药大学学报》2021年第10期2203-2208,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药局资助课题(编号:20194003,20193004)。

摘　　要：【目的】观察加减益经汤对环磷酰胺所致卵巢功能减退大鼠卵巢转化生长因子β(TGF-β)超家族相关成员的影响。【方法】将60只成年雌性SD大鼠随机分为空白组,模型组,加减益经汤高、中、低剂量组,补佳乐组,每组10只。除空白组,其余各组大鼠采用环磷酰胺75 mg/kg腹腔单次注射法构建卵巢功能减退模型。造模后2 h,加减益经汤高、中、低剂量组分别给予加减益经汤23.58、11.79、5.895 g·kg^(-1)·d^(-1)(生药剂量)灌胃,补佳乐组给予补佳乐混悬液0.09 mg·kg^(-1)·d^(-1)灌胃,模型组与空白组灌胃等体积蒸馏水,1次/日,连续28 d。模型组在造模后2周,其他治疗组在给药结束后,于动情间期处死。酶联免疫吸附分析(ELISA)检测血清抗苗勒氏管激素(AMH)、抑制素B(INHB)水平,免疫组织化学法检测卵巢中AMH、TGF-β1、转化生长因子β1受体(TGF-β1R)、骨形态发生蛋白15(BMP-15)的表达。【结果】与空白组比较,模型组大鼠血清AMH、INHB水平,卵巢组织中AMH、TGF-β1、TGF-β1R、BMP-15表达水平均降低(P<0.01);与模型组比较,加减益经汤高、中、低剂量组及补佳乐组大鼠血清AMH水平,卵巢组织中AMH、TGF-β1、TGF-β1R、BMP-15的表达水平均升高(P<0.05或P<0.01),加减益经汤高、中剂量组血清INHB水平明显升高(P<0.01)。【结论】加减益经汤可通过上调TGF-β超家族中AMH、INHB、TGF-β1、TGF-β1R、BMP-15的表达来改善环磷酰胺诱导的卵巢功能减退大鼠卵巢储备功能。Objective To observe the effect of modified Yijing Decoction(MYD)on transforming growth factor(TGF)-βsuperfamily-related members in rats with diminished ovarian reserve(DOR)induced by cyclophosphamide.Methods Sixty adult female SD rats were randomly divided into blank group,model group,low-,middle-and high-dose MYD groups,and Progynova group,10 rats in each group.Apart from the blank group,the rats in the other groups were induced into DOR model by one-off intraperitoneal injection of cyclophosphamide of 75 mg/kg.Hour 2 after modeling,low-,middle-and high-dose MYD groups were given intragastric administration of MYD at the rude drug dosage of 23.58,11.79,5.895 g·kg^(-1)·d^(-1),respectively,the Progynova group was given intragastric administration of Progynova suspension at the dosage of 0.09 mg·kg^(-1)·d^(-1),and the blank group and model group were given intragastric administration of the same volume of distilled water,once a day,the treatment lasting 28 days.The rats in the model group 2 weeks after modeling,and in the other groups after medication in estrum were sacrificed.The serum levels of anti-Müllerian hormone(AMH),inhibin B(INHB)were determined by enzyme-linked immunosorbent assay(ELISA),and the expression levels of AMH,TGF-β1,TGF-β1 receptor(TGF-β1R),bone morphogenetic protein 15(BMP-15)in ovarian tissue were detected by immunohistochemistry.Results Compared with the blank group,the serum levels of AMH and INHB,and the expression levels of AMH,TGF-β1,TGF-β1R,BMP-15 in ovarian tissue were decreased in the model group(P<0.01).Compared with the model group,the serum level of AMH,and the expression levels of AMH,TGF-β1,TGF-β1R,BMP-15 in ovarian tissue were increased in the high-,middle-and low-MYD groups,and Progynova group(P<0.05 or P<0.01),and the serum level of INHB was significantly enhanced in the high-and middle-MYD groups(P<0.01).Conclusion MYD is effective for improving ovarian function by upregulating the expression of AMH,INHB,TGF-β1,TGF-β1R,and BMP-15 in the TGF-βsuperfamily in ra

关 键 词：加减益经汤 卵巢功能减退 转化生长因子β超家族 转化生长因子Β1 转化生长因子Β1受体 抗苗勒氏管激素 抑制素B 骨形态发生蛋白15 大鼠 

分 类 号：R285.5[医药卫生—中药学]