基于稳定同位素比值的阳春砂产地判别分析  

Discrimination and Analysis of Geographical Origin of Amomum villosum Based on Stable Isotope Ratio

作　　者：钟慧怡 黄海波[1] 陈玉娥 覃挺红 ZHONG Hui-Yi;HUANG Hai-Bo;CHEN Yu-E;QIN Ting-Hong(School of Chinese Herbal Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006 Guangdong,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广州中医药大学学报》2021年第10期2224-2230,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：第四次全国中药资源普查项目(编号:广东省GZY-KJS-2019-003);2017年广东省岭南中药材保护资金——广东省首批8种中药材保护品种生产技术规范制定和完善专项(编号:粤财社〔2017〕60号)。

摘　　要：【目的】测定不同产地阳春砂的稳定同位素比值,探讨其地域特征及产地溯源的可能性。【方法】采用稳定同位素比质谱法测定阳春砂中同位素比率,结合正交偏最小二乘法判别分析(OPLS-DA),分别对广东、广西、云南3个省份及阳春、崇左、西双版纳、文山4个地域代表性产地进行初步判别,筛选出具有显著差异性的同位素,采用线性判别分析(LDA)方法建立阳春砂产地判别模型。【结果】不同产地的阳春砂中稳定同位素δ^(13) C、δD、δ^(15) N数值范围不同,具有一定的地域特征,其中δ13 C、δD为显著差异性同位素。建立的LDA判别模型对广东、广西和云南产地的判定准确率为76.9%,4个代表性产地的判定准确率为89.7%。【结论】利用稳定同位素比值结合多元统计方法可较好地进行阳春砂产地溯源,具有一定的可行性和应用价值。Objective To measure the stable isotope ratio of Amomum villosum from different habitats,and to explore its regional characteristics and possibility of geographic origin.Methods An isotope ratio-mass spectrometry was used to measure the stable isotope ratio of Amomum villosum,and combined with orthogonal partial least squares discrimination analysis(OPLS-DA)to preliminarily distinguish the 3 provinces such as Guangdong,Guangxi and Yunnan,and 4 regional representative habitats such as Yangchun,Chongzuo,Xishuangbanna and Wenshan to screen out the significant elements,and then linear discrimination analysis(LDA)was used to establish the discrimination models of Amomum villosum.Results The values ofδ^(13) C、δD、δ^(15) N in Amomum villosum from different habitats were different,and showed a specific character of geographic origin,among whichδ^(13) C andδD were significantly different isotopes.The correct discrimination rate of the established LDA model was 76.9%for the Amomum villosum samples from Guangdong,Guangxi and Yunnan,and 89.7%for the samples from 4 representative regions.Conclusion Determination of stable isotope ratio combined with multivariate statistical method applied is effective for tracing the geographic origin of Amomum villosum,which is of certain feasibility and application value.

关 键 词：阳春砂 产地溯源 稳定同位素 多元统计法 

分 类 号：R282.5[医药卫生—中药学]