髋关节脱位法联合液氮冷冻法建立兔早中期创伤性股骨头坏死动物模型观察  

Observation of Animal Model of Early-and Middle-Stage Traumatic Femoral Head Necrosis in Rabbits by Hip Joint Dislocation Method Combined with Liquid Nitrogen Freezing

作　　者：王禛 王上增[2] 余鹏 游明灿 WANG Zhen;WANG Shang-Zeng;YU Peng;YOU Ming-Can(Henan University of Chinese Medicine,Zhengzhou 450046 Henan,China;Henan Provincial Hospital of Traditional Chinese Medicine,Zhengzhou 450002 Henan,China;Shanghai Second Rehabilitation Hospital,Shanghai 201900,China;The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052 Henan,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中医院,河南郑州450002 [3]上海市第二康复医院,上海201900 [4]郑州大学第一附属医院,河南郑州450052

出　　处：《广州中医药大学学报》2021年第10期2236-2240,共5页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河南省中医管理局基金资助项目(编号:2016ZY2067);河南省中医管理局基金资助项目(编号:2019GDZX091);河南省中医管理局专项科学研究项目(编号:2019ZY2035);河南省中医管理局基金资助项目(编号:20-21ZY2010)。

摘　　要：【目的】观察髋关节脱位法联合液氮冷冻法建立兔股骨头坏死动物模型的效果。【方法】选取健康成年雄性日本大耳白兔28只,在无菌条件下使用外科手术法暴露股骨头,采用髋关节脱位法联合液氮冷冻法建立股骨头坏死模型。分别于造模后4、8周观察兔股骨头形态,并给予X线及MRI检查。在术后4、8周分别处死12、13只兔子,取造模侧股骨头观察骨小梁和髓腔的组织形态学变化。【结果】所有实验兔术后无明显感染迹象。造模后8周股骨头组织学结果发现空骨陷窝率增加,为8%~12%,超过正常兔股骨头软骨下区空骨陷窝率,符合股骨头坏死的判断标准,提示造模成功。【结论】采用髋关节脱位联合液氮冷冻法建立兔创伤性股骨头坏死模型,操作简单,动物死亡率较低,稳定可靠,可重复性好。Objective To observe the effect of establishing model of femoral head necrosis in rabbits by using hip joint dislocation method combined with liquid nitrogen freezing method.Methods Twenty-eight healthy adult male Japanese rabbits were selected to expose the femoral head by using surgical procedures to establish an model of femoral head necrosis by hip joint dislocation combined with liquid nitrogen freezing.Week 4,8 after the modeling,the morphological features of the femoral head of the rabbits was observed,and X-ray and MRI examination was carried out.Week 4,8 after the operation,12 and 13 rabbits were sacrificed respectively to take the femoral head of the modeling side to observe the histomorphological changes of trabecular bone and medullary cavity.Results All experimental rabbits showed no sign of infection after operation.Week 8 after modeling,the histological results of femoral head necrosis showed that the rate of hollow bone lacunae was increased,being 8%to 12%,more than that of normal rabbit femoral head subchondral area,meeting the criteria for femoral head necrosis,showing successful modeling.Conclusion Hip joint dislocation combined with liquid nitrogen freezing for establishing a rabbit model of traumatic femoral head necrosisis easy to operate,with low mortality rate,which is stable and reliable,and demonstrates good repeatability.

关 键 词：创伤性股骨头坏死 髋关节脱位 液氮冷冻 动物模型 兔 

分 类 号：R332[医药卫生—人体生理学]