GABA抑制腹主动脉瘤小鼠未成熟树突状细胞的迁移机制  

GABA inhibit the migration function of immature dendritic cells in abdominal aortic aneurysm mice

作　　者：杨颖[1] 罗晖 冯杰[1] 曾韡 YANG Ying;LUO Hui;FENG Jie;ZENG Wei(Cardiovascular Disease Laboratory,Department of Cardiology,Affiliated Hospital of North Sichuan Medical College;Department of Cardiothoracic Surgery,Nanchong Central Hospital,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院附属医院心内科·川北医学院附属医院心血管疾病研究室 [2]南充市中心医院心胸外科,四川南充637000

出　　处：《川北医学院学报》2021年第9期1249-1253,共5页Journal of North Sichuan Medical College

基　　金：四川省科技计划项目(2016JY0172);四川省卫生健康委科研课题(201716PJ121);国家自然科学基金青年基金项目(201881700417)。

摘　　要：目的:探讨γ-氨基丁酸(GABA)抑制腹主动脉瘤小鼠未成熟树突状细胞(iDCs)的迁移机制。方法:在腹主动脉瘤小鼠iDCs中转染GABA-A受体α5亚基SiRNA 48 h后,Western blot检测GABA-A受体α5亚基表达水平的变化。然后Transwell迁移实验检测GABA/GABA-A受体α5亚基对iDCs迁移能力的调控。激光共聚焦显微镜观察GABA/GABA-A受体α5亚基对iDCs伪足生成的影响。结果:在iDCs中转染GABA-A受体α5亚基SiRNA 48 h后,α5亚基的表达水平下降,差异有统计学意义(P<0.05)。GABA抑制iDCs的迁移,差异有统计学意义(P<0.05)。GABA抑制了iDCs伪足的形成。结论:GABA可能作用于GABA-A受体α5亚基抑制腹主动脉瘤小鼠iDCs的迁移和iDCs伪足的形成。Objective:To investigate the mechanism of GABA inhibiting migration function of immature dendritic cell(iDCs)in abdominal aortic aneurysm mice.Methods:48 hours after transfection of GABA-A receptorα5 subunit SiRNA in iDCs,the expression of GABA-A receptorα5 subunit was detected by Western blot.Then,the transwell migration experiment was performed to assess the regulation of iDCs migration ability by GABA/GABA-A receptor pathway.The regulatory effects of GABA/GABA-A receptor pathway on iDCs pseudopodia production was further analyzed by laser confocal microscopy.Results:48 hours after the transfection of GABA-A receptorα5 subunit SiRNA in iDCs,the expression ofα5 subunit significantly decreased(P<0.05).The migration of iDCs was significantly inhibited by GABA(P<0.05).GABA inhibited the formation of iDCs pseudofoot.Conclusion:GABA/GABA-A receptorα5 subunit inhibits the migration ability of iDCs and the formation of iDCs pseudofoot.

关 键 词：腹主动脉瘤 GABA 细胞迁移 未成熟树突状细胞 GABA受体 

分 类 号：R392.11[医药卫生—免疫学]