益脑心颗粒制备中7个活性成分转移率的研究  

Study on the transfer rate of seven active components in the preparation of Yinaoxin granules

作　　者：王鑫[1] 黄美玲 苏玉霞[1] 任晓东[3] 刘涛 陈明会[1] WANG Xin;HUANG Meiling;SU Yuxia;REN Xiaodong;LIU Tao;CHEN Minghui(Department of Pharmacy,Hanzhong People’s Hospital,Hanzhong 723000,China;Department of Critical Care Medicine,Hanzhong Railway Central Hospital;Department of Pharmacy,First Affiliated Hospital of Xi’an Jiaotong University)

机构地区：[1]汉中市人民医院药剂科,汉中723000 [2]汉中市铁路中心医院重症医学科 [3]西安交通大学第一附属医院药学部

出　　处：《山西医科大学学报》2021年第9期1217-1225,共9页Journal of Shanxi Medical University

摘　　要：目的建立高效液相色谱法(HPLC)法测定天麻、制何首乌、丹参、山楂、川芎药材与益脑心颗粒中腺苷、天麻素、没食子酸、5-羟甲基糠醛、二苯乙烯苷、迷迭香酸、丹酚酸B的含量,并探讨7种成分在药材至制剂间的转移率。方法采用Kromasil C 18(250 mm×4.6 mm,5μm)色谱柱;流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长270 nm;体积流量0.8 ml/min;柱温28℃;进样量10μl。检测7种成分在药材与制剂中的含量并计算7种成分从药材至制剂的转移率。结果腺苷、天麻素、没食子酸、5-羟甲基糠醛、二苯乙烯苷、迷迭香酸、丹酚酸B分别在质量浓度71.98-1799.59μg/ml(r=0.9998),22.39-559.63μg/ml(r=0.9996),2.00-499.59μg/ml(r=0.9999),3.20-799.98μg/ml(r=0.9999),12.00-299.88μg/ml(r=0.9997),1.60-400.25μg/ml(r=0.9998),80.01-2000.18μg/ml(r=0.9996)范围内,线性关系良好。7种成分的精密度、稳定性及重复性的RSD均小于2.5%,平均加样回收率97%-99%(RSD<2.0%)。在药材至制剂中腺苷、天麻素、没食子酸、5-羟甲基糠醛、二苯乙烯苷、迷迭香酸、丹酚酸B的转移率分别为67.49%,80.97%,69.75%,66.58%,53.24%,70.42%,62.28%。结论该含量测定方法操作简便、稳定可靠、重复性良好,可作为药材与益脑心颗粒中7个成分的定量方法;7个活性成分的转移率高,表明益脑心颗粒制备工艺有利于7个成分的转移。Objective To establish an HPLC method for determining the contents of adenosine,gastrodin,gallic acid,5-hydroxymethylfurfural,stilbene glucoside,rosmarinic acid and salvianolic acid B in Gastrodia elata,Polygoni multiflori radix praeparata,Salvia miltiorrhiza,Hawthorn,Chuanxiong rhizoma and Yinaoxin granules,and to explore the transfer rate from medicinal materials to preparations.Methods The kromasil C 18(250 mm×4.6 mm,5μm)column was used,with acetonitrile-0.1%phosphoric acid solution as a mobile phase in gradient elution,the detection wavelength was set at 270 nm.Volume flow rate was 0.8 ml/min,column temperature was set at 28℃,and the injection volume was 10μl.The contents of seven components in medicinal materials and preparations were detected,and the transfer rates of seven components from medicinal materials to preparations were calculated.Results For Adenosine,gastrodin,gallic acid,5-hydroxymethylfurfural,stilbene glycoside,rosmarinic acid and salvianolic acid B,the linear relationship was good in the range of 71.98-1799.59μg/ml(r=0.9998),22.39-559.63μg/ml(r=0.9996),2.00-499.59μg/ml(r=0.9999),3.20-799.98μg/ml(r=0.9999),12.00-299.88μg/ml(r=0.9997),1.60-400.25μg/ml(r=0.9998)and 80.01-2000.18μg/ml(r=0.9996),respectively.The RSDs of the precision,stability and repeatability of the seven components were less than 2.5%,the average recoveries were 97%-99%,and RSDs were less than 2.0%.The transfer rates of adenosine,gastrodin,gallic acid,5-hydroxymethylfurfural,stilbene glycoside,rosmarinic acid and salvianolic acid B were 67.49%,80.97%,69.75%,66.58%,53.24%,70.42%and 62.28%,respectively.Conclusion The method is simple,stable,reliable and reproducible,and can be used for the quantitative determination of seven active components in the medicinal materials and Yinaoxin granules.The transfer rates of seven active components are high,which indicates that the preparation process of Yinaoxin granules is conducive to the transfer of seven active components.

关 键 词：益脑心颗粒 高效液相色谱 活性成分 转移率 

分 类 号：R927.2[医药卫生—药学]