Complete Genome Sequencing and Analysis of Rehmannia Mosaic Virus Isolate from Shanxi Province  

作　　者：Wang De-fu Zhang Xi-mei Guo Shang Shen Shao-fei Long Dan-dan Li Ling-yu Niu Yan-bing 

机构地区：[1]College of Life Sciences,Shanxi Agricultural University,Taigu 030801,Shanxi,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China [3]Shanxi Institute for Functional Food,Shanxi Agricultural University,Taiyuan 030031,Shanxi,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2021年第3期58-65,共8页东北农业大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31772130);China Agriculture Research System(CARS-21)。

摘　　要：Using double-stranded RNA(dsRNA)technology and sequence-independent amplification(SIA),the molecular identification on infected Rehmannia glutinosa in the field with mosaic symptoms was performed and the whole-genome of the Rehmannia mosaic virus(ReMV)Shanxi isolate(ReMV-SX)was sequenced.Sequencing analysis showed that the virus that infected Rehmannia glutinosa was Rehmannia mosaic virus(ReMV).The full-length of the obtained ReMV-SX sequence(GenBank accession no.JX575184)was 6395 nt,containing four open reading frames(ORFs).The sequence homology analysis of the complete nucleotide sequence showed that ReMV-SX was 93.8%-97.0%homologous to ReMV in Tobamovirus subgroup Ⅰ,while only 49.8%-58.9%homologous to the isolates in subgroups Ⅱ and Ⅲ of the same genus.Phylogenetic analysis showed that ReMV-SX and ReMV-Henan formed a separate branch and had the closest genetic relationship.The results laid the foundation for ongoing researches in the taxonomic status and evolution of ReMV and for further investigating the pathogenic mechanism of ReMV infecting Rehmannia glutinosa.

关 键 词：Rehmannia mosaic virus(ReMV) Rehmannia glutinosa whole-genome amplification sequence analysis 

分 类 号：S432.4[农业科学—植物病理学]