高效液相色谱法同时测定化妆品中9种二苯甲酮类紫外吸收剂  

Simultaneous determination of nine benzophenone-type ultraviolet absorbents in cosmetics by high performance liquid chromatography

作　　者：鲁素雅 邢海艳 吴义春 金鹏[1] Lu Suya;Xing Haiyan;Wu Yichun;Jin Peng(Huai’an Institute for Food and Drug Control,Huai’an 223300,China)

机构地区：[1]淮安市食品药品检验所,淮安223005

出　　处：《化学分析计量》2021年第10期50-54,104,共6页Chemical Analysis And Meterage

摘　　要：建立同时测定化妆品中9种二苯甲酮类紫外吸收剂的高效液相色谱法。样品用乙腈溶解,经超声提取后,选择Waters Symmetry C18(250 mm×2.1 mm,5μm)色谱柱,以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,采用二极管阵列检测器检测,检测波长为322 nm,外标法定量。9种二苯甲酮类紫外吸收剂的质量浓度在0.25~50μg/mL范围内与色谱峰面积线性关系良好,相关系数为0.995~0.999,检出限为2.9~12.6μg/g,定量限为9.7~41.9μg/g。样品在3个不同浓度水平的加标回收率为89.84%~112.04%,测定结果的相对标准偏差为0.2%~7.7%(n=6)。该方法可用于化妆品中多种二苯甲酮类紫外吸收剂的同时检测。A method for simultaneous determining of nine benzophenone-type ultraviolet absorbents in cosmetics by high performance liquid chromatography was established.The samples were dissolved in acetonitrile and extracted by ultrasound,Waters Symmetry C18(250 mm×2.1 mm,5μm)was used as the chromatographic column.The mobile phase was acetonitrile-0.1%formic acid solution,gradient elution and diode array detector.The detection wavelength was 322 nm,and the external standard method was used for quantitative analysis.The mass concentration of the nine kinds of benzophenone-type ultraviolet absorbent had a good linear relationship with chromatographic peak area in the range of 0.25-50μg/mL,the correlation coefficients were 0.995-0.999,the detection limits were 2.9-12.6μg/g,and the quantitation limits were 9.7-41.9μg/g.The recoveries of samples at three different concentration levels were 89.84%-112.04%,and the relative standard deviations of determination results were 0.2%-7.7%(n=6).The method can be used for the simultaneous determination of variety of benzophenone-type ultraviolet absorbent in cosmetics.

关 键 词：高效液相色谱法 二苯甲酮 化妆品 紫外吸收剂 

分 类 号：O657.7[理学—分析化学]