康复新液治疗溃疡性结肠炎小鼠的效果  

The therapeutic effect of Kangfuxin liquid on ulcerative colitis mice

作　　者：金旭敏 孙洁 薛鹏鹏 董伟杰 陶信洁 徐荷林 JIN Xumin;SUN Jie;XUE Pengpeng;DONG Weijie;TAO Xinjie;XU Helin(Animal Laboratory Centre,Wenzhou Medical University,Wenzhou 325035,China;School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou 325035,China;The First School of Medicine,Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]温州医科大学实验动物中心,浙江温州325035 [2]温州医科大学药学院,浙江温州325035 [3]温州医科大学第一临床医学院,浙江温州325035

出　　处：《温州医科大学学报》2021年第9期699-704,712,共7页Journal of Wenzhou Medical University

基　　金：国家大学生创新创业训练计划项目(202010343019)。

摘　　要：目的:建立溃疡性结肠炎(UC)动物模型并评价康复新液(KFX)对UC的修复效果。方法:建立硫酸葡聚糖(DSS)诱导的UC小鼠模型,分为0.9%氯化钠溶液组(Saline组)、地塞米松灌肠液组(DXM组)和KFX组;评价UC小鼠治疗后症状缓解、脾肿胀、结肠长度、结肠组织髓过氧化物酶(MPO)水平,对比评价康复新液的治疗效果。对治疗后结肠组织进行HE染色、免疫组织化学染色、免疫荧光染色,比较3组小鼠结肠形态的修复效果。结果:与Saline组比,DXM组和KFX组UC小鼠腹泻和便血症状、脾肿胀明显缓解,结肠肿胀明显减轻,结肠长度增长,结肠组织MPO水平明显降低(P<0.05)。HE染色表明,与DXM组比,KFX组治疗后小鼠结肠黏膜上皮、隐窝、肌层黏膜和黏膜下层更为完整、各层分界分明,未见明显的肿胀与炎症细胞浸润,表明受损结肠形态有效修复。免疫组织化学染色表明,与DXM组比,KFX组炎症因子分泌明显减少(P<0.05),表明结肠组织有效修复。免疫荧光染色表明,与DXM组比,KFX组的紧密连接蛋白分泌明显有所增加(P<0.05),表明受损结肠组织黏膜屏障得到有效修复。结论:KFX能有效缓解UC症状并修复受损的结肠形态。Objective:To prepare Kangfuxin liquid(KFX)and Dexamethasone(DXM)enema and to evaluate its therapeutic effect on DSS induced ulcerative colitis mice.Methods:The dextran sulfate induced ulcerative colitis mice model was established and the model mice were divided into three groups:Saline group,DXM group and KFX group.The therapeutic effect of these treatment on UC mice was evaluated from aspects of symptom relief,spleen swelling,colon length,and MPO level of colon tissue.Besides,the morphology and mucosal barrier of colon were evaluated by HE staining,IHC staining and immunofluorescence staining respectively.Results:After DXM or KFX treatment,DSS induced UC mice had significantly reduced diarrhea,hematochezia,spleen swelling,colon swelling,colon length growth and MPO level in comparison with Saline group(P<0.05).Moreover,HE staining showed more complete mucosa epithelium layer,crypt,muscle layer mucosa and better-repaired submucosa after KFX treatment in comparison with DXM.No swelling or inflammatory cell infiltration in each layer of colon was observed in KFX group,indicating the effective morphological repair of the damaged colon.IHC staining showed that the secretion of inflammatory factors in KFX group was significantly reduced,compared with DXM group(P<0.05),indicating that colon tissue was effectively repaired.IF staining showed that the secretion of tight junction protein in KFX group was significantly increased(P<0.05),indicating that the damaged colonic mucosal barrier was effectively repaired.Conclusion:KFX oral liquid can effectively relieve UC symptoms and repair the damaged colon.

关 键 词：溃疡性结肠炎 康复新液 地塞米松 小鼠 

分 类 号：R94[医药卫生—药剂学]