毛发中7种常见合成大麻素类新精神活性物质的分析及应用  

Analysis and Application of Seven Common New Psychoactive Substances of Synthetic Cannabinoids in Hair

作　　者：施妍 周莉英 沈保华 陈航 刘梦曦 向平 刘伟 SHI Yan;ZHOU Li-ying;SHEN Bao-hua;CHEN Hang;LIU Meng-xi;XIANG Ping;LIU Wei(Shanghai Key Laboratory of Forensic Medicine,Key Laboratory of Forensic Science,Ministry of Justice,Shanghai Forensic Service Platform,Academy of Forensic Science,Shanghai 200063,China;School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110000,China)

机构地区：[1]司法鉴定科学研究院,上海市法医学重点实验室司法部,司法鉴定重点实验室上海市司法鉴定专业技术服务平台,上海200063 [2]沈阳药科大学药学院,辽宁沈阳110000

出　　处：《法医学杂志》2021年第4期479-485,492,共8页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(81971789);上海市社会发展科技攻关项目(19DZ1200600);司法部司法鉴定重点实验室资助项目;上海市法医学重点实验室资助项目(21DZ2270800);上海市司法鉴定专业技术服务平台资助项目(19DZ2292700)。

摘　　要：目的建立毛发中常见合成大麻素类新精神活性物质的超高效液相色谱-串联质谱(ultra-high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)测定方法。方法将20mg毛发加入1mL内标甲醇溶液,经冷冻研磨、超声提取后,提取物经ACQUITY UPLC HSS T3柱(100mm×2.1mm,1.8μm)分离,流动相A为含20mmol/L乙酸铵、0.1%甲酸、5%乙腈的水溶液,流动相B为乙腈,采用电喷雾离子源正离子模式,在多反应监测模式下采集数据。结果毛发中7种合成大麻素类物质在各自线性范围内线性关系良好(r>0.99),检出限为0.5~2pg/mg,定量限为1~5pg/mg,日内、日间精密度为0.1%~12.6%,日内、日间准确度为89.2%~110.7%,提取回收率为52.3%~93.3%,基质效应为19.1%~95.2%。结论建立的测定方法样品制备简便、灵敏度高,适用于法医学鉴定实践毛发中常见合成大麻素类新精神活性物质的定性定量分析。Objective To establish a detection method for common new psychoactive substances of synthetic cannabinoids in hair with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods In the 1 mL of internal standard methanol solution,20 mg hair was added.After cryogenic grinding and ultrasonic extraction,the extract was separated by ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8μm).The mobile phase A was aqueous solution that composed of 20mmol/L ammonium acetate,0.1%formic acid,and 5%acetonitrile.The mobile phase B was acetonitrile.Electrospray ionization source in positive ion mode was used for data acquisition in multi-reaction monitoring(MRM)mode.Results The seven common new psychoactive substances of synthetic cannabinoids in hair had a good linear relationship within their respective linear ranges(r>0.99),the limits of detection were 0.5-2 pg/mg,the limits of quantification were 1-5 pg/mg,the intra-day and inter-day precisions were 0.1%-12.6%,the intra-day and inter-day accuracies were 89.2%-110.7%,the recovery rates were 52.3%-93.3%,and the matrix effects were 19.1%-95.2%.Conclusion The established method has a simple sample preparation process and high sensitivity.It is suitable for qualitative and quantitative analysis of common new psychoactive substances of synthetic cannabinoids in hair.

关 键 词：法医学 毒物分析 超高效液相色谱法 串联质谱法 新精神活性物质 合成大麻素 2-[1-(4-氟丁基)-1H-吲唑-3-甲酰氨基]-3 3-二甲基丁酸甲酯 毛发 

分 类 号：DF795.1[医药卫生—法医学]