QuEChERS结合UPLC-MS/MS检测血液中3种色胺类新精神活性物质  

Determination of Three Types of New Psychoactive Tryptamines in Blood by QuEChERS Combined with UPLC-MS/MS

作　　者：侯伟 王燕燕[1] 张瑛 张蕾萍[3] 辛国斌[1] 覃仕扬[1] 王继芬[2] HOU Wei;WANG Yan-yan;ZHANG Ying;ZHANG Lei-ping;XIN Guo-bin;QIN Shi-yang;WANG Ji-fen(Key Laboratory of Forensic Toxicology,Ministry of Public Security,Beijing Public Security Bureau,Beijing 100192,China;School of Investigation,People’s Public Security University of China,Beijing 100038,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]法庭毒物分析公安部重点实验室北京市公安局,北京100192 [2]中国人民公安大学侦查学院,北京100038 [3]公安部物证鉴定中心,北京100038

出　　处：《法医学杂志》2021年第4期516-523,共8页Journal of Forensic Medicine

基　　金：法庭毒物分析公安部重点实验室(北京市公安局)开放基金资助项目(2019FTDWFX05)。

摘　　要：目的建立一种QuEChERS结合超高效液相色谱-串联质谱法(ultra-high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)快速筛查和检验人体血液中3种色胺类新精神活性物质——N,N-二烯丙基-5-甲氧基色胺(5-MeO-DALT)、N-甲基-N-异丙基-5-甲氧基色胺(5-MeO-MiPT)、N,N-二异丙基-5-甲氧基色胺(5-MeO-DiPT)的方法。方法分别考察了萃取剂种类、盐析剂种类及用量、吸附剂用量等条件对3种色胺类物质检验结果的影响。血液样品经QuEChERS方法处理后采用UPLCMS/MS法进行检测。结果人体血液中5-MeO-DALT、5-MeO-MiPT、5-MeO-DiPT分别在0.5~100、0.5~100、0.2~100ng/mL范围内具有良好的线性关系,相关系数均大于0.99,检出限为0.1~0.2ng/mL,回收率范围在84.86%~94.57%,日内及日间精密度良好。结论该方法简单、快速、易于操作、回收率高,适用于血液中色胺类物质的定性定量分析,能够为公安机关处理相关案件提供借鉴和参考。Objective To establish a method combining QuEChERS and ultra-high liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)for rapid screening and testing of three types of new psychoactive tryptamines in human blood:5-MeO-DALT,5-MeO-MiPT and 5-MeO-DiPT.Methods The effects of the type of extractant,the type and dosage of salting-out agent,and the dosage of adsorbent on the test results of the three tryptamines were investigated.Blood samples were processed by QuEChERS method and then determined by UPLC-MS/MS.Results The linear relationships of 5-MeO-DALT,5-MeO-MiPT and 5-MeO-DiPT in human blood were good in the range of 0.5-100,0.5-100 and 0.2-100 ng/mL,respectively,with their coefficients higher than 0.99.The limits of detection(LODs)were 0.1-0.2 ng/mg.The recoveries ranged from 84.86%to 94.57%.Intra-day and inter-day precisions were good.Conclusion The method is simple,rapid,easy to operate and has a high recovery.It is suitable for the qualitative and quantitative study of tryptamines in blood and can provide the reference for public security organs to deal with related cases.

关 键 词：法医学 毒物分析 新精神活性物质 色胺类 QUECHERS 超高效液相色谱-串联质谱法 N N-二烯丙基-5-甲氧基色胺 N-甲基-N-异丙基-5-甲氧基色胺 N N-二异丙基-5-甲氧基色胺 血液 

分 类 号：DF795.1[医药卫生—法医学]