16S rRNA基因测序技术分析胃窦糜烂患者的胃黏膜菌群特征  

Gastric Microbiota of Patients with Antrum Erosion by 16S rRNA Amplicon Sequencing Technique

作　　者：唐华英[1] 罗欣锦 邓志华[2] 覃艳春 叶广彬 TANG Huaying;LUO Xinjin;DENG Zhihua;QIN Yanchun;YE Guangbin(School of Preclinical Medicine,Youjiang Medical University for Nationalities,Baise 533000,China;Department of Gastroenterology/Affiliated Hospital,Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院基础医学院,广西百色533000 [2]右江民族医学院附属医院/消化内科,广西百色533000

出　　处：《信阳师范学院学报（自然科学版）》2021年第4期560-565,共6页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(32060018);广西自然科学基金项目(2020GXNSFAA259034);广西高校中青年教师基础能力提升项目(2019KY0580,2020KY13007);2020年国家级大学生创新创业训练计划项目(202010599006)。

摘　　要：提取胃窦糜烂患者的胃黏膜菌群基因组总DNA,进行高通量测序,结合数据分析其胃黏膜菌群特征.所有样品胃黏膜菌群在门分类水平上,其结构主要由变形菌门、厚壁菌门、拟杆菌门和放线菌门等组成;在属分类水平上,则由螺杆菌属、Gilliamella属、链球菌属和Snodgrassella属等优势菌属组成;其中YHP14、YHP15和YHP16样品的螺杆菌属极显著高于YHP11、YHP12和YHP13(P<0.001).此外,YHP14、YHP15和YHP16样品的菌群丰度和多样性均低于YHP11、YHP12和YHP13.幽门螺杆菌感染可改变胃窦糜烂患者的胃黏膜菌群结构,其诱导的慢性炎症是慢性胃窦糜烂发生、发展的机制之一.The total genome DNA was extracted from the gastric mucosal flora of patients with gastric antrum erosion,and the characteristics of the gastric mucosal flora were analyzed by high-throughput sequencing combined with data.At the phylum classification level,the gastric mucosal flora of all samples was mainly composed of Proteobacteria,Firmicutes,Bacteroidetes,and Actinobacteria.At the genus classification level,it was composed of Helicobacter,Gilliamella,Streptococcus and Snodgrassella.Among them,the Helicobacter of YHP14,YHP15 and YHP16 samples was significantly higher than that of YHP11,YHP12 and YHP13(P<0.001).In addition,the abundance and diversity of the flora of YHP14,YHP15 and YHP16 samples were lower than YHP11,YHP12 and YHP13.Helicobacter pylori infection can change the structure of gastric mucosal flora in patients with gastric antrum erosion,and the chronic inflammation induced by Helicobacter pylori infection is one of the mechanisms of the occurrence and development of chronic gastric antrum erosion.

关 键 词：16S rRNA 胃窦糜烂 胃黏膜菌群 幽门螺杆菌 

分 类 号：R537.3[医药卫生—内科学]