毛细管电泳-化学发光法测定牛奶中己烷雌酚、己烯雌酚和双烯雌酚  

Determination of Hexestrol,Dienestrol and Diethylstilbestrol in Milk by Capillary Electrophoresis with Chemiluminescence Detection

作　　者：王博[1] 白湧沨 翟一静 李增宁[2] 连靠奇 王玮 WANG Bo;BAI Yongfeng;ZHAI Yijing;LI Zengning;LIAN Kaoqi;WANG Wei(Handan University,Handan 056005,China;First Affiliated Hospital,Hebei Medical University,Shijiazhuang 050017,China;School of Public Health/Hebei Key Laboratory of Environment and Human Healt,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]邯郸学院,河北邯郸056005 [2]河北医科大学第一医院,河北石家庄050017 [3]河北医科大学公共卫生学院/环境与人群健康重点实验室,河北石家庄050017

出　　处：《信阳师范学院学报（自然科学版）》2021年第4期634-638,共5页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(81773430);河北省“三三三人才工程”资助项目(A202001114)。

摘　　要：结合毛细管电泳高分离效能和化学发光高灵敏度优势,建立了一种灵敏可靠的测定牛奶中己烷雌酚、己烯雌酚和双烯雌酚的毛细管电泳-化学发光新方法.结果表明:己烷雌酚、己烯雌酚及双烯雌酚能显著抑制Ag(Ⅲ)-鲁米诺化学发光体系的化学发光,且发光强度与样品浓度在一定范围内呈良好的线性关系.在优化实验条件下,己烷雌酚、己烯雌酚及双烯雌酚在15 min内完成分离.己烷雌酚、己烯雌酚及双烯雌酚的浓度分别在0.6~60、0.5~40、1~60 mg/L范围内与化学发光信号峰高呈良好线性关系,检出限分别为:0.57、0.35、0.80 mg/L.RSD分别为:2.38%、1.90%、1.3%.对实际样品进行加标检测,结果令人满意.A capillary electrophoresis(CE)coupling with chemiluminescence(CL)detection method for determining hexestrol,dienestrol and diethylstilbestrol was developed based on the enhanced CL intensity of the luminol and Ag(Ⅲ) in alkaline solution.Based on the fact that hexestrol,dienestrol and diethylstilbestrol can dramatically restrain the chemiluminescence intensity of Luminol-Ag(Ⅲ) complex chemiluminescence system,a good linear relationship between the chemiluminescence intensity and concentrations of analytes is develped.A good baseline separation of hexestrol,dienestrol and diethylstilbestrol is obtained within 15 min.The calibration curves are linear in the range of 0.6~60 mg/L for Hexestrol and 0.5~40 mg/L for diethylstilbestrol and 1~60 mg/L for dienestrol.The limits of detection of dexestrol,diethylstilbestrol and dienestrol are 0.57 mg/L,0.35 mg/L and 0.80 mg/L,respectively,with the relative standard deviation(RSD) of 2.38%,1.90% and 1.3%.The method can be applied to the separation and the detection of milk sample.The proposed method was applied to detect hexestrol,dienestrol and diethylstilbestrol in milk sample with satisfactory assay results.

关 键 词：毛细管电泳 化学发光 己烷雌酚 己烯雌酚 双烯雌酚 

分 类 号：O657.8[理学—分析化学]