Efficient Transformation of indica Rice Mediated by Agrobacterium and Generation of NcGDH Transgenic Genic Male-Sterile Rice with High Nitrogen Use Efficiency  

作　　者：LIU Cong TANGg Dongying ZHOU Zhengkun ZENG Hui HU Xiaochun TAN Vanning QIN Peng DENG Yong WU Jicai WANG Yan YANG Yuanzhu YUAN Dingyang LIU Xuanming LIN Jianzhong 

机构地区：[1]Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation,College of Biology,Hunan University,Changsha 410082,China [2]Academy of Seed Industry of Hunan Yahua,Changsha 410001,China [3]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125,China

出　　处：《Rice science》2021年第6期529-531,I0030-I0036,共10页水稻科学（英文版）

基　　金：supported by the National Science Foundation of China(Grant Nos.31571635 and 31871595);Hunan Provincial Important Science and Technology Specific Projects(Grant No.2018NK1010);Natural Science Foundation of Hunan Province,China(Grant No.2020JJ4004);Public Subject of State Key Laboratory of Hybrid Rice(Hunan Hybrid Rice Research Center)(Grant No.2019KF02);China Postdoctoral Science Foundation(Grant No.2020M682561).

摘　　要：Although some indica rice varieties have already been transformed by Agrobacterium,there is still great demand of developing transformation system for economically important indica varieties.Here,a fast and efficient method of Agrobacterium-mediated transformation for indica rice was developed,and an NcGDH transgenic genic male-sterile(GMS)indica rice 628S was generated using this method.The regenerated transgenic plantlets can be obtained within three months,and the transformation efficiency achieved about 17%.NcGDH transgenic 628S exhibited an enhanced capacity of ammonium assimilation and more effective panicles under low nitrogen conditions.Moreover the hybrid rice lines generated by NcGDH transgenic 628S showed 8%-27%yield advantage than those by the non-transgenic 628S.Taken together,these results provide an efficient transfbnnation method for indica rice and an opportunity to improve nitrogen use efficiency(NUE)and the heterosis of hybrid rice.

关 键 词：STERILE TRANSFORMATION TRANSFORMATION 

分 类 号：S511.21[农业科学—作物学]