Cloning and Characterization of Protein Prenyltransferase Alpha Subunit in Rice  

作　　者：WANG Tao LOU Lijuan LI Zeyu SHANG Lianguang WANG Quan 

机构地区：[1]Shenzhen Branch,Guangdong Laboratory for Lingnan Modem Agriculture/Genome Analysis Laboratory of Ministry of Agriculture and Rural Affairs/Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,China

出　　处：《Rice science》2021年第6期557-566,I0037,共11页水稻科学（英文版）

基　　金：supported by the Science Technology and Innovation Committee of Shenzhen Municipality of China(Grant Nos.JCYJ20170303154319837 and JCYJ20170412155447658);the Science Technology Innovation and Industrial Development of Dapeng New District,Shenzhen,China(Grant Nos.PT201901-18 and PT201901-20).

摘　　要：Protein prenylation plays a crucial role in plant development and stress response.We report the function of pren yltra nsferase a-sub unit in rice.Protein-protei n in teractions showed that the fam esyl-transferase(OsPFT)/geranylgeranyltransferase-l(OsPGGT l-a)protein interacted together with OsPFT-P and OsPGGT l-p.The a-and p-subunits of OsPFT formed a heterodimer for the transfer of a farnesyl group from famesyl pyrophosphate to the CaaX-box-containing peptide N-dansyl-GCVLS.Furthermore,the tissue expressi on patter ns of the OsPFT and OsPGGT I sub units were similar,and these sub units were localized in the cytoplasm and nucleus.Moreover,OsPFT/OsPGGT/-a-deletion homozygous rice mutants had a lethal phenotype,and the heterozygous mutants exhibited reduced pollen viability.These results indicated that prenylation plays an important role in rice development.

关 键 词：protein prenylation plant development RICE pollen viability 

分 类 号：S511[农业科学—作物学]