伊木萨克通过抑制复合应激勃起功能障碍大鼠模型阴茎组织中法尼酯X受体/含黄素单加氧酶3/氧化三甲胺通路促进勃起功能  

Yimusake promotes erectile function by inhibiting FXR/FMO3/TMAO pathway in penile tissue of ED rats under the compound stress

作　　者：靳瑾 田艳南 袁千茹 斯依提·阿木提 艾孜孜·热合曼 阿地力江·伊明 刘凤霞 JIN Jin;TIAN Yannan;YUAN Qianru;SIYITI Amuti;AIZIZI Reheman;ADILIJIANG Yiming;LIU Fengxia(Department of Human Anatomy,Collge of Basic Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang,China;College Student of Clinic Medicine(5+3)in 2016,Collge of First Clinic Medicine,Xinjiang Medical University,Urumqi 830011,Xinjiang,China)

机构地区：[1]新疆医科大学基础医学院人体解剖学教研室,乌鲁木齐830011 [2]新疆医科大学第一临床学院,乌鲁木齐830011

出　　处：《中国性科学》2021年第9期1-4,共4页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金(81860781);新疆医科大学第13期大学生创新训练计划(CX2018074)。

摘　　要：目的研究伊木萨克对复合应激勃起功能障碍(ED)大鼠模型阴茎组织中法尼酯X受体/含黄素单加氧酶3/氧化三甲胺(FXR/FMO3/TMAO)脂代谢通路的影响,并探讨其调控机制。方法选取2018年9月购自新疆医科大学实验动物中心的150只6周龄的雄性Sprag-Dawley大鼠作为研究对象。采用酶联免疫吸附试验法检测TMAO在正常对照组(n=30)、复合应激ED组(n=30)和伊木萨克给药组(n=30)血清中的浓度;采用免疫组化法检测FMO3、FXR1/2在三组阴茎组织中表达水平的变化。结果①复合应激ED组血清中TMAO浓度为(44.59±5.86)μg/mL,较正常对照组的(32.38±4.27)μg/mL升高37.71%,差异具有统计学意义(P<0.001);伊木萨克给药组血清中TMAO浓度为(37.68±4.15)μg/mL,较复合应激ED组下降16.37%,差异具有统计学意义(P<0.001)。②复合应激ED组阴茎组织中FMO3表达水平为(166.31±59.43),较正常对照组的(63.45±17.97)升高162.30%,差异具有统计学意义(P<0.001);伊木萨克给药组阴茎组织中FMO3表达水平为(78.53±65.69),较复合应激ED组降低52.78%,差异具有统计学意义(P<0.001)。③复合应激ED组阴茎组织中FXR1表达水平为(73.51±29.50),较正常对照组的(26.98±13.52)升高172.46%,差异具有统计学意义(P<0.001);伊木萨克给药组阴茎组织中FXR1表达水平为(44.64±14.37),较复合应激ED组降低39.27%,差异具有统计学意义(P<0.001)。复合应激ED组大鼠阴茎组织FXR2表达水平为(63.53±23.40),较正常对照组的(38.56±11.42)升高64.76%,差异具有统计学意义(P<0.001);伊木萨克给药组大鼠阴茎组织FXR2表达水平为(48.65±15.31),较复合应激ED组降低23.42%,差异具有统计学意义(P<0.001)。结论伊木萨克可抑制复合应激ED大鼠模型中FXR/FMO3/TMAO脂代谢通路的活化,对ED大鼠起到保护作用,其作用机制可能涉及抗氧化、降血脂作用。Objective To study the effect of Yimusake on the farnesoid X receptor/flavin-containing monooxygenase 3/trimethylamine-N-oxide(FXR/FMO3/TMAO)lipid metabolism pathway in penile tissue of the rat model of erectile dysfunction(ED)with complex stress,and explore its regulation mechanism.Methods 1506-week-old male Sprag-Dawley rats purchased from the Experimental Animal Center of Xinjiang Medical University in September 2018 were selected as the research objects.The serum concentrations of TMAO in normal control group(n=30),compound stress ED group(n=30)and Yimusake intervention group(n=30)were determined by enzyme-linked immunosorbent assay.The expression levels of FMO3 and FXR1/2 in penile tissues of the three groups were detected by immunohistochemistry.Results(1)The concentration of serum TMAO in the compound stress ED group(44.59±5.86)μg/mL was 37.71% higher than that in the normal control group(32.38±4.27)μg/mL(P<0.001).The serum TMAO concentration in the Yimusake intervention group(37.68±4.15)μg/mL was 16.37% lower than that in the compound stress ED group(P<0.001).(2)FMO3 expression in the penis was increased in the compound stress ED group(166.31±59.43)than the normal control group(63.45±17.97)by 162.30%(P<0.001).FMO3 expression in the penis was decreased in the Yimusake intervention group(78.53±65.69)than the compound stress ED group by 52.78%(P<0.001).(3)FXR1 expression in the penis was increased in the compound stress ED group(73.51±29.50)than the normal control group(26.98±13.52)by 172.46%(P<0.001).FXR1 expression in the penis was decreased in the Yimusake intervention group(44.64±14.37)than the compound stress ED group by 39.27%(P<0.001).FXR2 expression in the penis was increased in the compound stress ED group(63.53±23.40)than the normal control group(38.56±11.42)by 64.76%(P<0.001).FXR2 expression in the penis was decreased in the Yimusake intervention group(48.65±15.31)than ED group by 23.42%(P<0.001).Conclusions Yimusake can inhibit the activation of FXR/FMO3/TMAO lipid metabolism pathw

关 键 词：伊木萨克 勃起功能障碍 氧化三甲胺 含黄素单加氧酶3 法尼酯X受体 

分 类 号：R698[医药卫生—泌尿科学]