高危型HPV感染及CALCA基因启动子甲基化在宫颈病变检测中的临床价值  

Clinical value of high-risk HPV infection and CALCA promoter methylation in diagnosis of cervical lesions

作　　者：霍会蚕 王如意 李艳云 曲长萍 王宁 王琛 HUO Hui-can;WANG Ru-yi;LI Yan-yun;QU Chang-ping;WANG Ning;WANG Chen(Huaihe Hospital of Henan University,Kaifeng,Henan 475000,China;不详)

机构地区：[1]河南大学淮河医院妇产科,河南开封475000 [2]开封大学医学部,河南开封475000

出　　处：《中华医院感染学杂志》2021年第17期2628-2632,共5页Chinese Journal of Nosocomiology

基　　金：河南省科研基金资助项目(2016GH178)。

摘　　要：目的探讨高危型人乳头瘤病毒(HR-HPV)感染、降钙素基因相关肽α(CALCA)基因启动子甲基化在宫颈病变检测中的应用价值。方法选择2017年1月-2019年12月于河南大学淮河医院进行阴道镜病理组织活检的360例患者作为研究对象,根据阴道镜病理活检结果分为宫颈炎组、低度鳞状上皮内病变(LSIL)组、高度鳞状上皮内病变(HSIL)组、宫颈癌组,分析各组患者HR-HPV和低危型HPV(LR-HPV)感染情况,并检测宫颈病变组织中CALCA基因启动子区CpG位点甲基化情况,分析HR-HPV感染、CALCA基因启动子区CpG位点甲基化与宫颈癌临床特征的关系。结果四组患者HR-HPV阳性率比较差异有统计学意义(χ^(2)=137.201,P<0.001),其中宫颈癌组HR-HPV阳性率最高;四组患者LR-HPV阳性率比较差异无统计学意义(χ^(2)=4.123,P=0.248);四组CALCA基因启动子区CpG位点甲基化率比较差异具有统计学意义(χ^(2)=153.190,P<0.001),其中宫颈癌组甲基化率为80.00%(36/45);HR-HPV阳性宫颈癌患者临床分期、淋巴结转移与HR-HPV阴性患者比较差异有统计学意义(P<0.05);CALCA基因启动子区CpG位点甲基化宫颈癌患者肿瘤分化程度、临床分期、淋巴结转移与非甲基化患者比较差异有统计学意义(P<0.05)。结论宫颈癌组HR-HPV阳性率较高,HR-HPV感染及CALCA基因启动子区CpG位点甲基化与宫颈癌发生和发展有关,对临床宫颈癌诊断具有重要作用。OBJECTIVE To explore the value of high-risk human papillomavirus(HR-HPV)infection and calcitonin gene-related peptide alphaα(CALCA)promoter methylation in diagnosis of cervical lesions.METHODS Totally 360patients who underwent colposcopy pathological biopsy in Huaihe Hospital of Henan University from Jan 2017 to Dec 2019were recruited as the study subjects and divided into the cervicitis group,the lower-grade squamous intraepithelial lesion(LSIL)group,the high-grade squamous intraepithelial lesion(HSIL)group and the cervical cancer group according to the result of colposcopic biopsy.The prevalence rates of HR-HPV and LR-HPV infection were analyzed,the methylation of CpG locus in the promoter region of CALCA gene in the cervical lesion tissues was detected,and the association between the HR-HPV infection,methylation of CpG locus in the promoter region of CALCA gene and the clinical characteristics of cervical cancer was observed.RESULTS There was significant difference in the positive rate of HR-HPV among the four groups(χ^(2)=137.201,P<0.001),and the positive rate of HR-HPV of the cervical cancer group was the highest.There was no significant difference in the positive rate of LR-HPV among the four groups(χ^(2)=4.123,P=0.248).There was significant difference in the rate of methylation of CpG locus in the promoter region of CALCA gene among the four group(χ^(2)=153.190,P<0.001),and the rate of methylation of the cervical cancer group was 80.00%(36/45).There were significant differences in the clinical stage and lymphatic metastasis between the HR-HPV-positive patients and the HR-HPV-negative patients(P<0.05).There were significant differences in the degree of tumor differentiation,clinical stage and lymphatic metastasis between the patients with methylation of CpG locus in the promoter region of CALCA gene and the patients without methylation(P<0.05).CONCLUSIONThe positive rate of HR-HPV is relatively high among the cervical cancer patients.The HR-HPV infection and methylation of CpG locus in the promoter regi

关 键 词：高危型人乳头瘤病毒 降钙素基因相关肽α 甲基化 宫颈癌 宫颈上皮内瘤变 

分 类 号：R711.74[医药卫生—妇产科学]