宣城地区绝经后宫颈病变患者高危型HPV感染调查及C-myc基因表达  

Survey of high-risk HPV infection in postmenopausal women with cervical lesions in Xuancheng and amplification of C-myc gene

作　　者：钱慧 陈珉 牛多山 饶阳阳 郭芳 QIAN Hui;CHEN Min;NIU Duo-shan;RAO Yang-yang;GUO Fang(Xuancheng People′s Hospital,Xuancheng,Anhui 242100,China)

机构地区：[1]宣城市人民医院妇科,安徽宣城242100 [2]宣城市人民医院病理科,安徽宣城242100

出　　处：《中华医院感染学杂志》2021年第17期2648-2652,共5页Chinese Journal of Nosocomiology

基　　金：安徽省科研基金资助项目(2018376)。

摘　　要：目的探究宣城地区绝经后女性宫颈病变患者高危型人乳头瘤病毒(HPV)感染流行病学及C-myc基因扩增分析。方法选择2016年9月-2019年12月于宣城市人民医院妇科门诊就诊并接受妇科检查的宣城地区绝经期妇女作为研究对象,所有受试者均行宫颈薄层液基细胞学检查、HPV检测,异常患者行阴道镜及病理活检,采用荧光原位杂交法检测细胞C-myc基因扩增情况。结果入组1584例受试者共450例高危型HPV阳性,HPV感染率为28.41%,各亚型中HPV52、HPV16、HPV58检出率最高,分别为6.63%、6.00%、5.18%。入组1584例受试者中共303例存在宫颈上皮内瘤变(CIN),不同程度宫颈病变患者HPV感染率及混合感染率比较差异有统计学意义(χ^(2)=339.050,338.272;均P<0.001)。正常或炎性组、CINⅠ组、CINⅡ组、CINⅢ组、子宫颈癌组检出C-myc基因扩增表达情况比较差异有统计学意义(χ^(2)=763.406,P<0.001)。多因素Logistic分析结果,高危型HPV感染、C-myc基因扩增是宫颈病变发生的独立危险因素(P<0.05)。结论宣城地区绝经期妇女HPV感染率较高,HPV感染、C-myc基因扩增与宫颈病变发生及程度密切相关。OBJECTIVE To explore the epidemiological characteristics of high-risk human papilloma virus(HPV)infection in postmenopausal women with cervical lesions in Xuancheng and analyze the amplification of C-myc gene.METHODS The postmenopausal women who were treated and received gynecological examination in gynecology outpatient of Xuancheng People′s Hospital from Sep 2016to Dec 2019were recruited as the study subjects.All of the subjects underwent Thinprep cytology test and HPV examination,the abnormal patients underwent colposcopy an pathological biopsy,and the amplification of C-myc gene was detected by means of fluorescence in situ hybridization.RESULTS Of 1584subjects who were enrolled in the study,450were tested positive for high-risk HPV,and the incidence of HPV infection was 28.41%.The isolation rates of HPV52,HPV16and HPV58were 6.63%,6.00%and 5.18%,respectively.Among 1584subjects,303had cervical intraepithelial neoplasia(CIN),and there were significant differences in the incidence of HPV infection and the incidence of mixed infection among the patients with different degree of cervical lesions(χ^(2)=339.050,338.272;all P<0.001).There was significant difference in the amplification of C-myc gene among the normal or inflammation group,the CINⅠgroup,the CINⅡgroup,the CINⅢgroup and the cervical cancer group(χ^(2)=763.406,P<0.001).The result of multivariate logistic analysis showed that the high-risk HPV infection and amplification of C-myc gene were the independent risk factors for the cervical lesions(P<0.05).CONCLUSION The incidence of HPV infection is high among the postmenopausal women in Xuancheng.The HPV infection and amplification of C-mycgene are closely associated with the occurrence and degree of cervical lesions.

关 键 词：宣城地区 人乳头瘤病毒 宫颈病变 子宫颈癌 C-MYC基因 

分 类 号：R711.74[医药卫生—妇产科学]