艾灸“天枢穴”对溃疡性结肠炎大鼠结肠组织circRNA表达谱的影响  

Effects of moxibustion at‘Tianshu(ST 25)’on the circRNA expression profiles in colon from ulcerative colitis rats

作　　者：徐静[1] 李昆珊 汪迪 赵继梦[2] 陈珂旭 吴璐一[3] 王逸然 马喆[2] 钟蕊 黄艳[1,2] 吴焕淦[1,2] XU Jing;LI Kun-shan;WANG Di;ZHAO Ji-meng;CHEN Ke-xu;WU Lu-yi;WANG Yi-ran;MA Zhe;ZHONG Rui;HUANG Yan;WU Huan-gan(Yueyang Integrated Chinese and Western Medicine Hospital,Shanghai 200437,China;Shanghai Institute of Acupuncture-Moxibustion and Meridian,Shanghai 200030,China;Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437 [2]上海市针灸经络研究所,上海200030 [3]上海中医药大学,上海201203

出　　处：《中华中医药杂志》2021年第8期4669-4675,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81674074,No.81774405,No.81873372);上海中医药事业发展三年行动计划项目[No.ZY(2018-2020)-CCCX-2004-01];上海市卫生系统优秀人才培养计划(No.2018YQ11)。

摘　　要：目的:探讨艾灸“天枢穴”对溃疡性结肠炎大鼠环状RNA(circRNA)表达谱的影响。方法:将雄性SD大鼠随机分为对照组、模型组、隔药灸组、西药组,每组8只,通过观察各组大鼠疾病活动度(DAI)、结肠宏观损伤评分、结肠组织病理学变化及评分(CMDI)明确艾灸对溃疡性结肠炎的效应。每组取3只大鼠结肠组织进行高通量测序,获取差异表达的circRNAs,并进行生物信息学分析。结果:与正常组比较,模型组溃疡形成,结肠黏膜上皮不完整,腺体异常,杯状细胞显著减少,可见大量炎性细胞浸润,DAI、结肠宏观损伤评分及CMDI评分均显著增高(P<0.01);结肠组织差异表达的circRNAs有99个。与模型组比较,隔药灸组和西药组大鼠DAI指数、结肠宏观损伤评分及CMDI评分均显著降低(P<0.01),差异表达的circRNAs分别有99、76个。艾灸干预前后差异表达的circRNAs亲本基因与免疫炎症密切相关。结论:艾灸“天枢穴”可能通过影响结肠组织circRNA表达谱,作用其亲本基因而调节免疫相关通路,达到减轻DSS诱导UC大鼠结肠炎症反应之目的。Objective:To explore the influence of herb-partitioned moxibustion at‘Tianshu(ST 25)’on the circRNA expression profiles in rats with ulcerative colitis(UC).Methods:Male SD rats were randomly divided into a normal group,model group,herb-partitioned moxibustion group and western medicine group,8 for each group.Disease activity index(DAI),and colonic mucosa damage index(CMDI)were observed to reveal the effect of moxibustion on UC.Total RNA was extracted from 3 rats in each group,and chose differentially expressed circRNAs by RNA-seq and bioinformatics.Results:Compared with the normal group,ulceration was formed the intestinal mucosa was not intact,the gland structure was destroyed,and there was a large amount of inflammatory cell infiltration in model group.UC rats exhibited colonic mucosa damage,with a reduced number of glands and substantial inflammatory cell infiltration,DAI and CMDI were significantly increased in the model group(P<0.01);there were 99 differentially expressed circRNAs.Compared with the model group,DAI and CMDI were significantly decreased in the herb-partitioned moxibustion group and the western medicine group(P<0.01).There were 99 and 76 differentially expressed circRNAs in the herb-partitioned moxibustion group and the western medicine group,respectively.The pathways of the parental genes of differentially expressed circRNAs in the herb-partitioned moxibustion group were closely related to immune inflammation and carcinogenesis.Conclusion:The moxibustion at‘Tianshu(ST 25)’may regulate the circRNA expression profiles,act on the parental genes to regulate inflammatory signaling pathways,and attenuate inflammation in the colons of rats with DSS-induced UC.

关 键 词：溃疡性结肠炎 隔药灸 环状RNA表达谱 微小RNA 信号通路 互作分析 艾灸 生物信息学 

分 类 号：R245.8[医药卫生—针灸推拿学]