慢性胃炎不同病理阶段对舌苔菌门的影响  

Effects of different pathological stages of chronic gastritis on tongue coating bacterial phyla

作　　者：李莉[1] 王慧雯 丛军[1] 顾志坚[1] 郭春荣 蒋海洋 李娜[2] 孙祝美[2] LI Li;WANG Hui-wen;CONG Jun;GU Zhi-jian;GUO Chun-rong;JIANG Hai-yang;LI Na;SUN Zhu-mei(Department of Gastroenterology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China;School of Basic Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院消化科,上海200021 [2]上海中医药大学基础医学院,上海201203

出　　处：《中华中医药杂志》2021年第8期4964-4968,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：首届全国名中医传承工作室建设项目(No.MZYGZS-2017003);上海市科学技术委员会科研计划项目(No.18401971400);上海中医药大学预算内科研项目(No.2019LK067)。

摘　　要：目的:探讨慢性胃炎不同病理阶段对舌苔菌门的影响。方法:采用16S rRNA基因测序技术对慢性胃炎患者舌苔菌门进行研究,实验分为炎症组(30例)、单纯萎缩组(20例)和萎缩伴肠化组(30例)。结果:与其他两组比较,萎缩伴肠化组Alpha指数Sobs、Ace、Chao均显著升高(P<0.05);在舌苔菌群的构成上,3组优势菌门的组成(拟杆菌门、厚壁菌门、变形菌门、梭杆菌门、放线菌门、Saccharibacteria)基本相似。与其他两组比较,萎缩伴肠化组特有微量菌门较多;在舌苔菌门的相对丰度方面,3组优势菌门中厚壁菌门相对丰度有显著性差异(P<0.05),而6种微量菌门(软壁菌门、酸杆菌门、蓝细菌门、绿弯菌门、疣微菌门、芽单胞菌门)在3组中存在显著性差异(P<0.05);舌苔微生物构成的网络在3组之间也存在差异:炎症组舌苔菌群整体网络是不连通的,可形成局部小的网络连接,而单纯萎缩组能形成一个相对松散的网络结构,萎缩伴肠化组参与网络构建的微量菌门增加,网络更加趋于密集。结论:慢性胃炎不同病理阶段对舌苔菌门具有一定的影响,特别在萎缩伴肠上皮化生阶段,舌苔菌群生物多样性和微量菌门数目显著增多,这说明随着疾病的发生发展,患者的舌苔菌群也发生相应的改变,提示舌苔菌群与胃部病理变化关系密切,口腔-胃在人体形成微生物共生的微环境,为舌苔-口腔菌群-肠道菌群的联系提供支持。Objective:To investigate the effects of different pathological stages of chronic gastritis on tongue coating flora.Methods:16S rRNA gene sequencing method was used to study tongue coating flora in chronic gastritis patients.The patients were divided into inflammation group(30 cases),atrophic group(20 cases)and atrophy with intestinal metaplasia group(30 cases).Results:Compared with the other two groups,the Alpha index Sobs,Ace and Chao of atrophic with intestinal metaplasia group were significantly increased(P<0.05).The composition of dominant bacterial phyla(Bacteroidea,Firmicutes,Proteobacteria,Fusobacteria,Actinobacteria,Saccharicteria)was similar in three groups.Compared with the other two groups,there were more unique microbial phyla in atrophic with intestinal metaplasia group.There were significant differences in the relative abundances of the dominant phylum Firmicutes(P<0.05),while the microbial phyla(Tenericutes,Cyanobacteria,Acidobacteria,Chloroflexi,Verrucomicrobia and Gemmatimonadetes)among the three groups(P<0.05).There were also differences among the three groups in the composition of network.The whole network of tongue coating flora in inflammation group was not connected,which could form local small network connection,while atrophy group could form a relatively loose network structure,while atrophy with intestinal metaplasia group had more microbial phyla involved in network construction,and the network tended to be more dense.Conclusion:Different pathological stages of chronic gastritis has a certain influence on tongue coating flora.The biodiversity and microbial phyla number of the tongue coating flora increases significantly,especially in the atrophic with intestinal metaplasia stage.It indicates that the tongue coating flora of the patients change correspondingly with the development of the disease and is closely related to the pathological changes of the stomach.The oral and stomach form the microbial symbiosis in the human body providing the support for the relationship among the tongue co

关 键 词：慢性胃炎 舌苔菌群 16S rRNA基因测序技术 病理阶段 菌门 

分 类 号：R259[医药卫生—中西医结合]