慈姑多糖对异烟肼/利福平联用致HepG2细胞损伤HO-1和GCLC的影响  

Effects of Sagittaria Sagittifolia polysaccharide on HepG2 cell hepatic damage HO-1 and GCLC induced by isoniazid/rifampicin

作　　者：刘红双 刘文胜 王晶 张烯 热依拉·吐尔逊 杨亚洁 廖艳 LIU Hong-shuang;LIU Wen-sheng;WANG Jing;ZHANG Xi;Reyila•Tuerxun;YANG Ya-jie;LIAO Yan(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102448,China;China International Engineering Consulting Corporation,Beijing 100048,China;Baizhifang Community Health Service Center,Beijing 100054,China)

机构地区：[1]北京中医药大学中医学院,北京102488 [2]中国国际工程咨询有限公司,北京100048 [3]北京市西城区白纸坊社区卫生服务中心,北京100054

出　　处：《中华中医药杂志》2021年第8期4977-4981,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81503633);北京中医药新奥奖励基金项目(No.1000062720044/004);北京中医药大学校级科研纵向发展基金项目(No.2018-ZXFZJJ-030)。

摘　　要：目的:研究慈姑多糖(SSP)对异烟肼(INH)和利福平(RFP)联用致HepG2细胞损伤中血红素加氧酶-1(HO-1)和谷氨酰-L-半胱氨酸连接酶催化亚单位(GCLC)酶含量、基因及蛋白表达影响。方法:采用ELISA检测HepG2细胞中HO-1和GCLC含量变化;采用定量RT-PCR技术检测HepG2细胞中HO-1、GCLC mRNA的表达;采用Western Blot法检测HepG2细胞中HO-1和GCLC蛋白的表达。结果:与正常对照组比较,模型组HepG2细胞中HO-1和GCLC含量、mRNA及蛋白表达显著增加(P<0.05);与模型组比较,SSP最佳给药组中HO-1和GCLC mRNA、蛋白表达显著升高(P<0.05)。结论:SSP可能通过促进HepG2细胞中HO-1及GCLC含量、mRNA及蛋白的表达,发挥其对INH/RFP联用致肝损伤的保护作用。Objective:To study the effects of Sagittaria Sagittifolin polysaccharide(SSP)on the content of HO-1 and GCLC enzymes,genes and proteins expression in HepG2 cell injury caused by the combined use of isoniazid and rifampicin(INH/RFP).Methods:Enzyme-linked immunoassay(ELISA)was used to detect the changes of HO-1 and GCLC content in HepG2 cells;quantitative RT-PCR was used to detect the expression of HO-1 and GCLC content mRNA in HepG2 cells;Western Blot method was used to detect HepG2 cells HO-1 and GCLC protein expression.Results:Compared with the normal control group,the model group could increase the HO-1 and GCLC content,mRNA and protein expression in HepG2 cells(P<0.05).Compared with the model group,the mRNA and protein expression of HO-1 and GCLC in the SSP best administration group was significantly increased(P<0.05).Conclusion:SSP may play a protective role in the liver injury induced by the INH/RFP through promoting the content of HO-1 and GCLC mRNA and protein expression in HepG2 cells.

关 键 词：慈姑多糖 异烟肼/利福平联用 肝保护 血红素加氧酶-1 谷氨酰-L-半胱氨酸连接酶催化亚单位 机制 

分 类 号：R285.5[医药卫生—中药学]