五加减正气散含药血清对Toll样受体3信号通路及相关炎症因子表达的影响  

Effects of serum containing Wujiajian Zhengqi Powder on TLR3 signaling pathway and related inflammatory factors expression

作　　者：王伟[1,2] 陈翰翰[1] 陈婷[3] 曹志群[3] WANG Wei;CHEN Han-han;CHEN Ting;CAO Zhi-qun(The Affiliated Hospital of Shandong University of TCM,Jinan 250014,China;School of Medicine,Shandong University,Jinan 250012,China;First Clinical College of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)

机构地区：[1]山东中医药大学附属医院,济南250014 [2]山东大学临床医学院,济南250012 [3]山东中医药大学第一临床学院,济南250014

出　　处：《中华中医药杂志》2021年第8期4991-4993,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81904128);山东省博士后创新项目(No.202003069);山东省中医药科技发展计划项目(No.2019-0159)。

摘　　要：目的:研究五加减正气散(WJJZQS)含药血清对To ll样受体3(TLR3)信号通路及相关炎症因子表达的影响。方法:制备染毒细胞模型(RV SA11株,MA104细胞)。Wistar大鼠按体质量随机分为空白组,中成药组,西药组及WJJZQS大、中、小剂量组,按剂量灌胃相应药物,2次/d,连续给药3d,于末次灌胃1h后眼眶取血。检测各含药血清作用染毒细胞后,TLR3信号通路及相关炎症因子的表达。结果:与染毒组比较,WJJZQS大、中剂量组能显著下调染毒细胞白介素(IL)-6、IL-1β、肿瘤坏死因子-α(TNF-α)的高表达,上调干扰素β(IFN-β)的低表达(P<0.05,P<0.01);WJJZQS小剂量组对IL-1β、TNF-α也有一定的调控作用(P<0.05);WJJZQS大、中剂量组可显著下调TLR3、TRIF mRNA及蛋白的过表达(P<0.01,P<0.05),但对IRF3 mRNA及蛋白调控作用不明显。结论:WJJZQS含药血清可通过调控TLR3信号通路关键蛋白及下游炎症因子途径发挥一定的抗病毒作用。Objective:To study the effects of the serum containing Wujiajian Zhengqi Powder(WJJZQS)on TLR3 signaling pathway and the expression of related inflammatory factors.Methods:Preparation of infected cell model(RV SA11 strain,MA104 cells).Wistar rats were randomly divided into 6 groups according to body weight:blank group,Chinese patent medicine group,Western medicine group and WJJZQS large,medium and small dose groups.Gavage the appropriate drugs according to the dose,twice a day.The drug was administered continuously for 3 days,and blood was collected from the eye socket after 1h of the last gavage.The expression of TLR3 signaling pathway and related inflammatory factors were detected after each medicated serum acted on infected cells.Results:Compared with the infected group,WJJZQS large and medium dose groups could significantly down-regulate the high expression of IL-6,IL-1βand TNF-α,up-regulated the low expression of IFN-βin infected cells(P<0.05,P<0.01).The small dose group also had certain regulatory effects on IL-1βand TNF-α(P<0.05).WJJZQS large and medium dose groups could significantly down-regulate the high expression of TLR3,TRIF mRNA and overexpression of protein(P<0.05,P<0.01).However,IRF3 mRNA and protein were not significantly regulated.Conclusion:The serum containing WJJZQS can play a certain antiviral effect by regulating the key proteins of TLR3 signaling pathway and downstream inflammatory factor pathway.

关 键 词：五加减正气散 含药血清 Toll样受体3信号通路 炎症因子 调控影响 

分 类 号：R285.5[医药卫生—中药学]