益气温阳护卫汤对哮喘大鼠Th17细胞IL-6-STAT3信号通路的影响  

Effects of Yiqi Wenyang Huwei Decoction on IL-6-STAT3 signaling pathway of Th17 cells in asthmatic rats

作　　者：喻强强[1] 鲍梦婕 余涛[2] 余建玮[1] 孙朋[1] 叶超[1] 薛汉荣[1] YU Qiang-qiang;BAO Meng-jie;YU Tao;YU Jian-wei;SUN Peng;YE Chao;XUE Han-rong(Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China;Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学附属医院,南昌330006 [2]江西中医药大学,南昌330004

出　　处：《中华中医药杂志》2021年第8期4994-4997,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81760801,No.81960802,No.82060858);江西省自然科学基金项目(No.20181BAB205085);江西省教育厅科学技术研究项目(No.GJJ170742);江西省中医药管理局科技计划项目(No.2019A014)。

摘　　要：目的:探究益气温阳护卫汤对哮喘大鼠辅助性T细胞17(Th17)细胞白介素-6(IL-6)-信号转导及转录激活因子3(STAT3)信号通路的影响。方法:按照随机方法,将144只SPF级雌性SD大鼠分为正常对照(A)组,模型(B)组,益气温阳护卫汤低、中、高剂量(C、D、E)组,地塞米松(F)组。卵蛋白(OVA)-氢氧化铝溶液腹腔致敏,雾化吸入OVA激发,F组灌服1mg/kg地塞米松,C、D、E组分别灌服1、2、4g/mL益气温阳护卫汤,各组分别于1、2、4周处死大鼠每组8只,检测BALF中Th17细胞比例和肺组织IL-6、STAT3 mRNA相对表达量。结果:与A组比较,各组同时段Th17细胞比例,IL-6、STAT3 mRNA相对表达量均升高(P<0.05);与B组比较,各药物组同时段均下调(P<0.05),D、E、F组下调较C组显著(P<0.05)。各药物组第2、4周Th17细胞比例及IL-6、STAT3 mRNA相对表达量较第1周显著下调(P<0.05),F组第2周STAT3 mRNA表达除外。IL-6 mRNA和STAT3 mRNA表达水平呈正相关。结论:益气温阳护卫汤可能通过下调IL-6、STAT3 mRNA表达,调控IL-6-STAT3信号通路影响Th17细胞分化达到抑制哮喘气道炎症的目的。Objective:To explore the effects of Yiqi Wenyang Huwei Decoction on IL-6-STAT3 signaling pathway of Th17 cells in asthmatic rats.Methods:A total of 144 SPF female SD rats were randomly divided into normal control group(A),model group(B),Yiqi Wenyang Huwei Decoction low,medium and high dose groups(C,D,E)and dexamethasone group(F).Ovalbumin(OVA)-aluminum hydroxide solution was sensitized intraperitoneally and stimulated by aerosol inhalation,Group F was treated with 1mg/kg dexamethasone,and groups C,D and E were treated with 1,2 and 4g/mL Yiqi Wenyang Huwei Decoction.Eight rats in each group were killed at 1,2 and 4 weeks,respectively.The proportion of Th17 cells in BALF and the relative expression of IL-6 and STAT3 mRNA in lung tissue were detected.Results:Compared with group A,the proportion of Th17 cells and the relative expression of IL-6 and STAT3 mRNA in each group were increased at the same time(P<0.05);Compared with group B,all giving drug groups were down regulated at the same time(P<0.05),and the down regulation in group D,E and F was more significant than that in group C(P<0.05).The proportion of Th17 cells and the relative expression of IL-6 and STAT3 mRNA in each drug group at 2 and 4 weeks were significantly lower than those at 1 week(P<0.05),except the expression of STAT3 mRNA in group F at 2 weeks.There was a positive correlation between IL-6 mRNA and STAT3 mRNA.Conclusion:Yiqi Wenyang Huwei Decoction can inhibit the airway inflammation of asthma by decreasing the expression of IL-6 and STAT3 mRNA,regulating IL-6-STAT3 signaling pathway and affecting the differentiation of Th17 cells.

关 键 词：支气管哮喘 益气温阳护卫汤 TH17细胞 IL-6-STAT3信号通路 MRNA 

分 类 号：R285.5[医药卫生—中药学]