益气清热膏对氨基核苷嘌呤霉素肾病综合征大鼠模型肾组织Wnt/β-catenin信号通路表达的影响  

Effects of Yiqi Qingre Cream on Wnt/β-catenin signaling pathway in renal tissues of puromycin aminonucleoside nephrosis rat model

作　　者：杨丽平[1] 刘咏梅[1] 占永立[1] 刘慧洁 孙雪艳 冒慧敏[1] 马放[1] 李平[4] YANG Li-ping;LIU Yong-mei;ZHAN Yong-li;LIU Hui-jie;SUN Xue-yan;MAO Hui-min;MA Fang;LI Ping(Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Shunyi Hospital of Beijing Traditional Chinese Medicine Hospital,Beijing 101300,China;Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]北京中医医院顺义医院,北京101300 [3]首都医科大学附属北京中医医院,北京100010 [4]中日友好医院,北京100029

出　　处：《中华中医药杂志》2021年第8期5029-5032,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81102588,No.81473614,No.81973657);中央级公益性科研院所基本科研业务费专项(No.ZZ13-YQ-025)。

摘　　要：目的:探讨益气清热膏对氨基嘌呤霉素肾病综合征模型Wnt/β-catenin信号通路相关分子表达的影响。方法:采用RT-PCR法及Western Blot法检测PAN模型造模后第3、10、15天大鼠肾组织中wnt4、wnt5a、β-catenin、GSK-3β、FEN的基因和蛋白表达情况。结果:造模后第15天,与模型组比较,益气清热膏可以抑制PAN大鼠模型wnt4、β-catenin、GSK-3β和FEN蛋白的表达(P<0.05,P<0.01),并且随着治疗时间延长,益气清热膏作用靶点逐渐增多。结论:益气清热膏可以降低PAN大鼠模型肾组织wnt4、β-catenin、GSK-3β、FEN的基因和蛋白表达,这可能是其降低蛋白尿、修复足细胞损伤的作用机制之一。Objective:To explore the effects of Yiqi Qingre Cream by investigating alterations of the molecule of Wnt/β-catenin signaling pathway in puromycin aminonucleoside nephrosis(PAN)rat model.Methods:RT-PCR and Western Blot analysis were conducted to detect the expressions of wnt4,wnt5a,β-catenin,GSK-3β,FEN of renal tissues were detected on the 3rd,10th and 15th day after PAN modeling.Results:Compared with model group,the protein expression levels of wnt4,β-catenin,GSK-3βand FEN was inhibited by Yiqi Qingre Cream(P<0.05,P<0.01)with the extension of treatment time,the target of Yiqi Qingre Cream gredually increased.Conclusion:Yiqi Qingre Cream can inhibit wnt4,β-catenin,GSK-3β,and FEN expression in PAN model,which may be related with its therapeutic mechanisms of reducing proteinuria and alleviating podocyte damage.

关 键 词：益气清热膏 蛋白尿 足细胞损伤 WNT/Β-CATENIN信号通路 机制 氨基核苷嘌呤霉素肾病综合征 动物模型 

分 类 号：R285.5[医药卫生—中药学]