微小RNA-143-3p对胶质瘤细胞增殖、迁移和侵袭的影响  

Effect of microRNA-143-3p on proliferation,migration and invasion of glioma cells

作　　者：张涛[1] 宋晋[2] ZHANG Tao;SONG Jin(Department of Neurology,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,Hubei Province,China;Department of Psychiatry,Wuhan Mental Health Center,Wuhan 430014,Hubei Province,China)

机构地区：[1]华中科技大学同济医学院附属武汉中心医院神经外科,湖北武汉430014 [2]武汉市精神卫生中心精神科,湖北武汉430014

出　　处：《中国临床药理学杂志》2021年第18期2440-2442,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究微小RNA-143-3p(microRNA-143-3p,miR-143-3p)通过调节基质金属蛋白酶2(Matrix metalloproteinase-2,MMP2)对胶质瘤细胞增殖,迁移和侵袭的影响。方法体外培养胶质瘤U87 MG细胞,并随机分为miR-NC组和miR-143-3p mimic组;miR-NC组为转染阴性对照寡核苷酸的U87 MG细胞,miR-143-3p mimic组为转染miR-143-3p模拟物的U87 MG细胞。以细胞计数试剂盒-8(Cell Counting Kit-8,CCK-8)检测胶质瘤U87 MG细胞存活率,以划痕实验检测胶质瘤U87 MG细胞迁移能力,以Transwell实验检测胶质瘤U87 MG细胞侵袭能力,以蛋白质印迹(Western blot)法检测胶质瘤U87 MG细胞中MMP2表达水平。结果48 h时,miR-NC组和miR-143-3p mimic组的细胞增殖率分别为(94.33±7.59)%和(71.43±7.01)%;72 h时,miR-NC组和miR-143-3p mimic组的细胞增殖率分别为(93.45±8.11)%和(68.31±6.51)%;miR-NC组和miR-143-3p mimic组的细胞迁移率分别为(42.03±5.72)%和(21.33±1.98)%;细胞侵袭数目为62.09±8.35和41.46±6.27;MMP2的表达量分别为1.01±0.10和0.68±0.07;以上指标,miR-143-3p mimic组与miR-NC组相比,差异均有统计学意义(均P<0.05)。结论miR-143-3p通过MMP2抑制胶质瘤细胞增殖,迁移和侵袭。Objective To study the effect of microRNA-143-3 p(miR-143-3 p)on glioma cell proliferation,migration and invasion by regulating matrix metalloproteinase-2(MMP2).Methods Glioma U87 MG cells were cultured in vitro,and randomly divided into miR-NC and miR-143-3 p mimics groups;miR-NC group was transfected with negative control oligonucleotides and the miR-143-3 p mimics group was transfected with miR-143-3 p mimics.Cell counting kit-8(CCK-8)assay was performed to detect the proliferation ability of glioma U87 MG cells.Wound healing assay was conducted to detect the migration ability of glioma U87 MG cells.Transwell assay was conducted to detect the invasion ability of glioma U87 MG cells.Western blot assay was used to detect the expression of MMP2 in glioma U87 MG cells.Results At 48 h,the cell proliferation rates of the miR-NC and miR-143-3 p mimic group were(94.33±7.59)%and(71.43±7.01)%,respectively;the cell proliferation rates at 72 h were(93.45±8.11)%and(68.31±6.51)%,respectively;the cell migration rate were(42.03±5.72)%and(21.33±1.98)%;the numbers of invasion cells were 62.09±8.35 and 41.46±6.27,respectively;the expressions of MMP2 in cells were 1.01±0.10 and 0.68±0.07,respectively.The differences between the miR-143-3 p mimic group and the miR-NC group were statistically significant(all P<0.05).Conclusion miR-143-3 p inhibits glioma cell proliferation,migration and invasion through MMP2.

关 键 词：miR-143-3p 基质金属蛋白酶2 胶质瘤 增殖 转移 

分 类 号：R97[医药卫生—药品]