病理组织芽生菌感染的特殊染色方法分析  

Analysis of special staining methods for blastomyces

作　　者：谢文林[1] 唐翠兰 连家燕 Xie Wenlin;Tang Cuilan;Lian Jiayan(Department of Pathology,the Seventh Affiliated Hospital of Sun Yat-sen University,Shenzhen 518107,China;Department of Pathology,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第七医院病理科,深圳518107 [2]中山大学附属第一医院病理科,广州510080

出　　处：《中国组织化学与细胞化学杂志》2021年第3期287-289,共3页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的分析比较显示真菌的4种特殊染色方法,旨在寻找一种显示芽生菌并适合于临床病理诊断的染色方法。方法对病理石蜡组织芽生菌感染标本进行PAS染色、PASM染色、抗酸染色及爱尔新蓝染色(pH2.5),光学显微镜下观察染色效果。结果HE染色切片上见多数圆形、厚壁、棕色的硬壳小体,PAS染色呈红色,PASM染色呈黑色,抗酸染色个别菌体呈红色,爱尔新蓝染色呈阴性。结论PAS染色和PASM染色均可用于显示芽生菌,但改良后的PAS染色时间最短,结构清晰、对比明显,是检测芽生菌效果最佳的染色方法。Objective To pick out the most suitable method for clinical-pathological diagnosis of blastomyces by comparing four special staining methods of fungus.Methods Pathological paraffin tissues infected with blastomyces were stained with PAS staining,PASM staining,acid-fast staining and Alisin blue staining(pH2.5),respectively.The dyeing effect was observed by light microscopy.Results Thick-walled round bodies with hard brown shells were observed on HE-stained sections.These bodies were stained red by PAS staining,black by PASM staining,partially red by acid-fast staining,and negative by Alisin blue staining.Conclusion Both PAS and PASM staining can be used to show blastomyces.The modified PAS staining,which requires short staining time while provides clear structure and contrast,is the best staining method for blastomyces.

关 键 词：芽生菌 PAS染色 六胺银染色 抗酸染色 爱尔新蓝染色 

分 类 号：R372[医药卫生—病原生物学]