急性热应激对鹅组织中NLRP3炎性小体基因表达的影响  

Effect of acute heat stress on NLRP3 inflammasome gene expression in goose tissues

作　　者：杨晨 萧显鑫 罗沛 邓志超 苏曼娜 陈世健 付新亮 曹楠 刘文俊 YANG Chen;XIAO Xianxin;LUO Pei;DENG Zhichao;SU Manna;CHEN Shijian;FU Xinliang;CAO Nan;LIU Wenjun(College of Animal Science and Technology,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Guangdong Province Key Laboratory of Waterfowl Healthy Breeding,Guangzhou 510225,China)

机构地区：[1]仲恺农业工程学院动物科技学院,广东广州510225 [2]广东省水禽健康养殖重点实验室,广东广州510225

出　　处：《仲恺农业工程学院学报》2021年第3期1-5,11,共6页Journal of Zhongkai University of Agriculture and Engineering

基　　金：广东省自然科学基金(2018A030313630);国家重点研发计划(2018YFE0128200);广州市科技计划(201804010412)资助项目.

摘　　要：本试验旨在研究NLRP3炎性小体在鹅热应激损伤中的作用.将27只雏鹅分为A、B和C 3组,对照组A组环境温度设为25℃,热应激组B组和C组分别在38℃和40℃环境下热处理1 h.采集各组雏鹅的脑、肝脏、脾脏、肺脏、空肠等组织,将A组和C组的组织制作切片进行病理学观察,并提取各组所有组织的RNA、反转录后通过荧光定量PCR法对NLRP3信号途径相关基因及下游炎症因子表达水平进行检测,并对A、B、C各组雏鹅静脉采血,用ELISA对血清中IL-1β的含量进行检测.组织切片结果显示,40℃热应激组的肝脏、脾脏、肺脏、空肠等组织出现明显的显微结构损伤和炎症细胞聚集增多.荧光定量结果显示热应激促进了HSP70和HSP90基因的表达,38℃热应激组NLRP3、ASC、Caspase-1在脾中表达水平显著高于对照组(P<0.05),IL-1β在肺组织中的表达量显著高于对照组(P<0.05),血清中IL-1β的含量也显著升高(P<0.05).试验表明热应激导致雏鹅组织炎症的发生,NLRP3炎性小体可能参与热应激处理导致的脾脏和肺脏的炎症损伤过程.The aim of this study was to investigate the role of NLRP3 inflammasome in heat stress injury in geese.Twenty-seven goslings were divided into three groups:A,B and C.The ambient temperature of group A(control group)was set at 25℃,and the heat treatment of group B and C was conducted at 38℃and 40℃for 1 h respectively.The brain,liver,spleen,lung,jejunum tissues of gosling in each group were collected.The tissues of group A and group C were sliced for pathological observation,and RNA was extracted from all tissues in each group.After reverse transcription,the expression levels of NLRP3 signaling pathway related genes and downstream inflammatory factors were detected by fluorescent quantitative PCR,and the A,B,C groups of gosling venous blood collection,using ELISA to detect the concentration of serum IL-1β.The results of tissue sections showed that the liver,spleen,lung,jejunum tissues in the 40℃ heat stress group showed obvious microstructure damage and increased accumulation of inflammatory cells.Fluorescence quantitative results showed that heat stress promoted the expression of HSP70 and HSP90 genes.The expression levels of NLRP3,ASC and Caspase-1 in spleen in the 38℃ stress group were significantly higher than those in control group(P<0.05).The expression levels of IL-1βin lung were significantly higher than those in control group(P<0.05).The concentration of IL-1βin serum also increased significantly(P<0.05).The results showed that heat stress induced tissue inflammation of gosling,and NLRP3 inflammasome may participate in the inflammatory injury process of spleen and lung induced by heat stress.

关 键 词：鹅 热应激 NLRP3炎性小体 损伤 

分 类 号：S835[农业科学—畜牧学]