活血软坚通络方对大鼠冠脉微血管血栓及内皮损伤因子水平的影响  

Influences of Chinese medicinal with actions of invigorating blood circulation,softing scleroma and firming collaterals prescription on coronary microvascular thrombosis and endothelial injury factors in rats

作　　者：张萌 李明 张莉 张明 苗鑫 李元民 Zhang Meng;Li Ming;Zhang Li;Zhang Ming;Miao Xin;Li Yuanmin(The Second Affiliated Hospital of Shandong First Medical University,Taian 271000,China)

机构地区：[1]山东第一医科大学第二附属医院,山东泰安271000

出　　处：《山东第一医科大学（山东省医学科学院）学报》2021年第9期657-661,共5页Journal of Shandong First Medical University ＆ Shandong Academy of Medical Sciences

基　　金：山东省中医药科技发展计划(2017-253);山东等自然科学基金(ZR2017 MH097);山东省医药卫生科技发展计划(2017WS424)。

摘　　要：目的研究中药活血软坚通络方对大鼠冠状动脉微血管血栓及内皮损伤因子水平的影响。方法将60只健康Wistar大鼠,采用随机数字表法分成模型组、尼可地尔组、中药组、假手术组(对照组)。模型组选择向大鼠左心室注射月桂酸钠建立大鼠冠状动脉微血管血栓及内皮损伤动物模型,用药干预组分别给予尼可地尔1.5 mg·(kg·d)^(-1)、中药15 g·(kg·d)^(-1),假手术组、模型组给予等量的蒸馏水灌服。28天后观察大鼠心肌切片病理改变及冠状动脉微血管血栓阻塞情况,并检测血清前列腺素Ⅰ_(2)(PGI_(2))、血栓素A_(2)(TXA_(2))。结果与假手术组比较,造模各组经左心室注射月桂酸钠导致冠脉微血管血栓形成及心肌病理损伤;用药28天,与模型组比较,中药组可减轻微血管栓塞,改善心肌病理损伤,增加PGI_(2),减少TXA_(2);与尼可地尔组相比,中药组在改善微血管血栓及减轻内皮损伤因子方面未见明显差别。结论利用月桂酸钠造模可引起大鼠冠脉微血管血栓形成及内皮损伤;中药组可减轻冠脉微血管栓塞并改善微血管病变内皮功能;与尼可地尔组相比,中药组未见明显优势。Objective:To investigate the effect of Chinese medicinal with actions of Invigorating blood circulation,softing scleroma and firming collaterals prescription on coronary microvascular thrombosis and endothelial injury factor in rats.Methods:60 Wistar rats were randomLy divided into:Sham operation group,Model group,Nicorandil group and Chinese medicine group.The sham operation group was used as the control group.The model was established by injecting sodium laurate into the left ventricle of rats to establish rat models of coronary microvascular hrombosis and endothelial function injury.The drug group was given Nicorandil 1.5 mg·(kg·d)^(-1),Traditional Chinese Medicine prescription 15 g·(kg·d)^(-1),Sham operation group and Model group were given the same amount of distilled water.After the 28th day,treatment was selected as the observation point to observe the myocardial pathological changes and coronary microvascular thrombosis occlusion,and to detect the serum levels of ProstaglandinⅠ_(2)(PGI_(2))and Thromboxane A_(2)(TXA_(2)).Results:Compared with the sham operation group,the left ventricular injection of sodium laurate in the model group led to coronary microvascular thrombosis and myocardial tissue pathological damage;After 28 days of medication,compared with the model group,the traditional Chinese medicine group could reduce microvascular embolism,improve myocardial pathological damage,increase serum prostaglandinⅠ_(2)(PGI_(2))and reduce thromboxane A_(2)(TXA_(2));Compared with the nicorandil group,there was no significant difference in the improvement of microvascular thrombosis and the reduction of endothelial injury factor in the traditional Chinese medicine group.Conclusion:Sodium laurate can cause coronary microvascular thrombosis and endothelial injury in rats.For 28 days,the traditional Chinese medicine group could reduce the pathological state of coronary microvascular embolization and improve the endothelial function of microvascular lesions.Compared with Nicordil,there was no obvious advant

关 键 词：冠脉微血管 活血软坚通络 微血管血栓 内皮损伤因子 月桂酸钠 PGI_(2)/TXA_(2) 

分 类 号：R242[医药卫生—中医临床基础]