桦木酸对人胃癌MGC-803细胞增殖的影响  

Effect of betulinic acid on proliferation of human gastric cancer MGC-803 cell line

作　　者：刘久鹏 邵淑丽[1,2] 何孟奇 黄鑫 张伟伟 张珍珠[1,2] 陈亚楠 LIU Jiu-peng;SHAO Shu-li;HE Meng-qi;HUANG Xin;ZHANG Wei-wei;ZHANG Zhen-zhu;CHEN Ya-nan(College of Life Science,Agriculture and Forestry,Qiqihar University;Heilongjiang Key Laboratory of Resistance Genetic Engineering and the Protection of Cold Region Biodiversity,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006 [2]抗性基因工程与寒地生物多样性保护黑龙江省重点实验室,黑龙江齐齐哈尔161006

出　　处：《中国应用生理学杂志》2021年第3期257-261,共5页Chinese Journal of Applied Physiology

基　　金：黑龙江省教育厅基本业务专项重点项目(135109104);黑龙江省教育厅基本业务专项(粮头食尾)(LTSW201737);黑龙江省省属高等学校基本科研业务费科研项目(植物性食品加工技术特色学科专项,YSTSXK201809);齐齐哈尔大学2019年研究生创新科研项目(YJSCX2019054)。

摘　　要：目的:探究不同浓度桦木酸对人胃癌MGC-803细胞增殖的影响。方法:将人胃癌MGC-803细胞分成4组,每组设置3个复孔,对照组细胞为加入浓度为0μg/ml的桦木酸实验组细胞分别加入终浓度为10、20、30μg/ml的桦木酸,各组细胞在含5%的CO 2培养箱中孵育48 h后,使用吉姆萨染色法和台盼蓝拒染法检测桦木酸对细胞克隆形成率和生长抑制率的影响;EdU法和流式细胞术分别检测细胞增殖和细胞周期变化;qRT-PCR和Western blot检测细胞周期调控因子CCNB1、CCND1的表达。结果:与对照组相比,人胃癌MGC-803的克隆形成率显著降低(P<0.01),生长抑制率明显升高,细胞增殖能力显著下降(P<0.01);各实验组细胞随着桦木酸浓度的增加G1期细胞所占比例逐渐降低,而S期细胞数量显著增多(P<0.01);细胞周期调控因子CCNB1、CCND1的mRNA和蛋白表达水平均显著降低(P<0.01),30μg/ml的桦木酸处理组的表现最佳。结论:在终浓度为10~30μg/ml的范围内,桦木酸能够降低人胃癌MGC-803细胞增殖,抑制细胞生长,下调CCNB1和CCND1的表达将人胃癌MGC-803细胞阻滞于G0/G1期。Objective:To investigate the effect of betulinic acid on the proliferation of human gastric cancer MGC-803 cells in vitro.Methods:Human gastric cancer MGC-803 cells were divided into 4 groups,each with 3 multiple holes.Control cells add betulinic acid at a concentration of 0μg/ml,and the other three experimental groups were added with final concentration of 10,20,30μg/ml Betulinic acid respectively.Cells in each group were incubated in a 5%CO 2 incubator for 48 hours,and the Giemsa staining method and trypan blue exclusion method were used to detect the effect of betulinic acid on the cell clone formation rate and growth inhibition rate;EdU method and flow cytometry were used to detect cell proliferation and cell cycle changes;qRT-PCR and Western blot were used to detect the expressions of cell cycle regulators CCNB1 and CCND1.Results:Compared with the control group,the clone formation rate of human gastric cancer MGC-803 cells was significantly reduced(P<0.01),the growth inhibition rate was significantly increased,and the cell proliferation ability was significantly decreased(P<0.01);with the increase of betulinic acid concentration in each experimental group the proportion of cells in the G1 phase was gradually decreased,and the number of cells in S phase was increased significantly(P<0.01);the mRNA and protein expression levels of cell cycle regulators CCNB1 and CCND1 were decreased significantly,and the 30μg/ml betulinic acid treatment group performed best.Conclusion:At a final concentration of 10~30μg/ml,betulinic acid can reduce the proliferation of human gastric cancer MGC-803 cells,inhibit cell growth,and down-regulate the expression of CCNB1 and CCND1 to block human gastric cancer MGC-803 cells in the S phase.

关 键 词：桦木酸 MGC-803细胞 细胞增殖 细胞周期调控因子 

分 类 号：Q291[生物学—细胞生物学]